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7c90

From Proteopedia

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Current revision

Crystal structure of Cytochrome CL from the marine methylotrophic bacterium Methylophaga aminisulfidivorans MPT (Ma-CytcL)

Structural highlights

7c90 is a 4 chain structure with sequence from Methylophaga aminisulfidivorans MP. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.13Å
Ligands:	, , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

F5SWR8_9GAMM

Publication Abstract from PubMed

Cytochrome cL (CytcL) is an essential protein in the process of methanol oxidation in methylotrophs. It receives an electron from the pyrroloquinoline quinone (PQQ) cofactor of methanol dehydrogenase (MDH) to produce formaldehyde. The direct electron transfer mechanism between CytcL and MDH remains unknown due to the lack of structural information. To help gain a better understanding of the mechanism, we determined the first crystal structure of heme c containing CytcL from the aquatic methylotrophic bacterium Methylophaga aminisulfidivorans MP(T) at 2.13 A resolution. The crystal structure of CytcL revealed its unique features compared to those of the terrestrial homologues. Apart from Fe in heme, three additional metal ion binding sites for Na(+), Ca(+), and Fe2(+) were found, wherein the ions mostly formed coordination bonds with the amino acid residues on the loop (G93-Y111) that interacts with heme. Therefore, these ions seemed to enhance the stability of heme insertion by increasing the loop's steadiness. The basic N-terminal end, together with helix alpha4 and loop (G126 to Y136), contributed positive charge to the region. In contrast, the acidic C-terminal end provided a negatively charged surface, yielding several electrostatic contact points with partner proteins for electron transfer. These exceptional features of Ma-CytcL, along with the structural information of MDH, led us to hypothesize the need for an adapter protein bridging MDH to CytcL within appropriate proximity for electron transfer. With this knowledge in mind, the methanol oxidation complex reconstitution in vitro could be utilized to produce metabolic intermediates at the industry level.

Crystal structure of Cytochrome cL from the aquatic methylotrophic bacterium Methylophaga aminisulfidivorans MP(T).,Ghosh S, Dhanasingh I, Ryu J, Kim SW, Lee SH J Microbiol Biotechnol. 2020 Jul 3. pii: jmb.2006.06029. doi:, 10.4014/jmb.2006.06029. PMID:32627749^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ghosh S, Dhanasingh I, Ryu J, Kim SW, Lee SH. Crystal structure of Cytochrome cL from the aquatic methylotrophic bacterium Methylophaga aminisulfidivorans MP(T). J Microbiol Biotechnol. 2020 Jul 3. pii: jmb.2006.06029. doi:, 10.4014/jmb.2006.06029. PMID:32627749 doi:http://dx.doi.org/10.4014/jmb.2006.06029

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7c90"

Categories: Large Structures | Methylophaga aminisulfidivorans MP | Dhanasingh I | Ghosh S | Lee SH

@@ Line 3: / Line 3: @@
 <StructureSection load='7c90' size='340' side='right'caption='[[7c90]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7c90]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methylophaga_aminisulfidivorans_mp Methylophaga aminisulfidivorans mp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C90 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C90 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7c90]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylophaga_aminisulfidivorans_MP Methylophaga aminisulfidivorans MP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C90 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.13&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MAMP_01204 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1026882 Methylophaga aminisulfidivorans MP])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c90 OCA], [http://pdbe.org/7c90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c90 RCSB], [http://www.ebi.ac.uk/pdbsum/7c90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c90 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c90 OCA], [https://pdbe.org/7c90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c90 RCSB], [https://www.ebi.ac.uk/pdbsum/7c90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c90 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/F5SWR8_9GAMM F5SWR8_9GAMM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 7c90" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
 == References ==
 <references/>
@@ Line 22: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Methylophaga aminisulfidivorans mp]]
+[[Category: Methylophaga aminisulfidivorans MP]]
-[[Category: Dhanasingh, I]]
+[[Category: Dhanasingh I]]
-[[Category: Ghosh, S]]
+[[Category: Ghosh S]]
-[[Category: Lee, S H]]
+[[Category: Lee SH]]
-[[Category: Electron transport]]
-[[Category: Marine bacterium]]
-[[Category: Methanol oxidation system]]
-[[Category: Methylophaga aminisulfidivorans mpt]]

7c90

From Proteopedia

Current revision

Crystal structure of Cytochrome CL from the marine methylotrophic bacterium Methylophaga aminisulfidivorans MPT (Ma-CytcL)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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