7cby
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Structure of FOXG1 DNA binding domain bound to DBE2 DNA site== |
| - | <StructureSection load='7cby' size='340' side='right'caption='[[7cby]]' scene=''> | + | <StructureSection load='7cby' size='340' side='right'caption='[[7cby]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7cby]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CBY FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.646Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cby OCA], [https://pdbe.org/7cby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cby RCSB], [https://www.ebi.ac.uk/pdbsum/7cby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cby ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Disease == | ||
| + | [https://www.uniprot.org/uniprot/FOXG1_HUMAN FOXG1_HUMAN] FOXG1 syndrome due to 14q12 microdeletion;14q11.2 microduplication syndrome. The disease is caused by variants affecting the gene represented in this entry. | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FOXG1_HUMAN FOXG1_HUMAN] Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.<ref>PMID:12657635</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Forkhead box G1 (FOXG1) is a transcription factor mainly expressed in the brain that plays a critical role in the development and regionalization of the forebrain. Aberrant expression of FOXG1 has implications in FOXG1 syndrome, a serious neurodevelopmental disorder. Here, we report the crystal structure of the FOXG1 DNA-binding domain (DBD) in complex with the forkhead consensus DNA site DBE2 at the resolution of 1.6 A. FOXG1-DBD adopts a typical winged helix fold. Compared to those of other FOX-DBD/DBE2 structures, the N-terminus, H3 helix and wing2region of FOXG1-DBD exhibit differences in DNA recognition. The FOXG1-DBD wing2 region adopts a unique architecture composed of two beta-strands that differs from all other known FOX-DBD wing2 folds. Mutation assays revealed that the disease-causing mutations within the FOXG1-DBD affect DNA binding, protein thermal stability, or both. Our report provides initial insight into how FOXG1 binds DNA and sheds light on how disease-causing mutations in FOXG1-DBD affect its DNA-binding ability. | ||
| + | |||
| + | Structural basis for DNA recognition by FOXG1 and the characterization of disease-causing FOXG1 mutations.,Dai S, Li J, Zhang H, Chen X, Guo M, Chen Z, Chen Y J Mol Biol. 2020 Oct 12. pii: S0022-2836(20)30583-0. doi:, 10.1016/j.jmb.2020.10.007. PMID:33058871<ref>PMID:33058871</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7cby" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[FOX 3D structures|FOX 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Chen YH]] |
| + | [[Category: Dai SY]] | ||
| + | [[Category: Li J]] | ||
Current revision
Structure of FOXG1 DNA binding domain bound to DBE2 DNA site
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Categories: Homo sapiens | Large Structures | Chen YH | Dai SY | Li J
