7cdz

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of 2019-nCoV nucleocapsid N-terminal domain (NTD) protein

Structural highlights

7cdz is a 4 chain structure with sequence from Severe acute respiratory syndrome coronavirus 2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCAP_SARS2 Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Publication Abstract from PubMed

COVID-19, caused by SARS-CoV-2, has resulted in severe and unprecedented economic and social disruptions in the world. Nucleocapsid (N) protein, which is the major structural component of the virion and is involved in viral replication, assembly and immune regulation, plays key roles in the viral life cycle. Here, we solved the crystal structures of the N- and C-terminal domains (N-NTD and N-CTD) of SARS-CoV-2 N protein, at 1.8 and 1.5 A resolution, respectively. Both structures show conserved features from other CoV N proteins. The binding sites targeted by small molecules against HCoV-OC43 and MERS-CoV, which inhibit viral infection by blocking the RNA-binding activity or normal oligomerization of N protein, are relatively conserved in our structure, indicating N protein is a promising drug target. In addition, certain areas of N-NTD and N-CTD display distinct charge distribution patterns in SARS-CoV-2, which may alter the RNA-binding modes. The specific antigenic characteristics are critical for developing specific immune-based rapid diagnostic tests. Our structural information can aid in the discovery and development of antiviral inhibitors against SARS-CoV-2 in the future.

Structures of the SARS-CoV-2 nucleocapsid and their perspectives for drug design.,Peng Y, Du N, Lei Y, Dorje S, Qi J, Luo T, Gao GF, Song H EMBO J. 2020 Oct 15;39(20):e105938. doi: 10.15252/embj.2020105938. Epub 2020 Sep , 11. PMID:32914439^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Peng Y, Du N, Lei Y, Dorje S, Qi J, Luo T, Gao GF, Song H. Structures of the SARS-CoV-2 nucleocapsid and their perspectives for drug design. EMBO J. 2020 Oct 15;39(20):e105938. doi: 10.15252/embj.2020105938. Epub 2020 Sep , 11. PMID:32914439 doi:http://dx.doi.org/10.15252/embj.2020105938

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7cdz"

@@ Line 3: / Line 3: @@
 <StructureSection load='7cdz' size='340' side='right'caption='[[7cdz]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7cdz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CDZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7cdz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CDZ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cdz OCA], [https://pdbe.org/7cdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cdz RCSB], [https://www.ebi.ac.uk/pdbsum/7cdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cdz ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cdz OCA], [https://pdbe.org/7cdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cdz RCSB], [https://www.ebi.ac.uk/pdbsum/7cdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cdz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2]] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
+[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: 2019-ncov]]
 [[Category: Large Structures]]
-[[Category: Gao, G F]]
+[[Category: Severe acute respiratory syndrome coronavirus 2]]
-[[Category: Peng, Y]]
+[[Category: Gao GF]]
-[[Category: Qi, J]]
+[[Category: Peng Y]]
-[[Category: Song, H]]
+[[Category: Qi J]]
-[[Category: Covid-19]]
+[[Category: Song H]]
-[[Category: Ntd]]
-[[Category: Nucleocapsid]]
-[[Category: Rna binding]]
-[[Category: Viral protein]]

7cdz

From Proteopedia

Current revision

Crystal structure of 2019-nCoV nucleocapsid N-terminal domain (NTD) protein

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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