7cp1
From Proteopedia
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==Crystal structure of isocitrate lyase in complex with succinate and itaconate== | ==Crystal structure of isocitrate lyase in complex with succinate and itaconate== | ||
| - | <StructureSection load='7cp1' size='340' side='right'caption='[[7cp1]]' scene=''> | + | <StructureSection load='7cp1' size='340' side='right'caption='[[7cp1]], [[Resolution|resolution]] 2.58Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CP1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7cp1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CP1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cp1 OCA], [https://pdbe.org/7cp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cp1 RCSB], [https://www.ebi.ac.uk/pdbsum/7cp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cp1 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.58Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ITN:2-METHYLIDENEBUTANEDIOIC+ACID'>ITN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cp1 OCA], [https://pdbe.org/7cp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cp1 RCSB], [https://www.ebi.ac.uk/pdbsum/7cp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cp1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ACEA_MYCTU ACEA_MYCTU] Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | During the glyoxylate cycle, isocitrate lyases (ICLs) catalyze the lysis of isocitrate to glyoxylate and succinate. Itaconate has been reported to inhibit an ICL from Mycobacterium tuberculosis (tbICL). To elucidate the molecular mechanism of ICL inhibition, we determined the crystal structure of tbICL in complex with itaconate. Unexpectedly, succinate and itaconate were found to bind to the respective active sites in the dimeric form of tbICL. Our structure revealed the active site architecture as an open form, although the substrate and inhibitor were bound to the active sites. Our findings provide novel insights into the conformation of tbICL upon its binding to a substrate or inhibitor, along with molecular details of the inhibitory mechanism of itaconate. | ||
| + | |||
| + | Heterogeneous multimeric structure of isocitrate lyase in complex with succinate and itaconate provides novel insights into its inhibitory mechanism.,Kwon S, Chun HL, Ha HJ, Lee SY, Park HH PLoS One. 2021 May 5;16(5):e0251067. doi: 10.1371/journal.pone.0251067., eCollection 2021. PMID:33951112<ref>PMID:33951112</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7cp1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Mycobacterium tuberculosis H37Rv]] | ||
[[Category: Kwon S]] | [[Category: Kwon S]] | ||
[[Category: Park HH]] | [[Category: Park HH]] | ||
Current revision
Crystal structure of isocitrate lyase in complex with succinate and itaconate
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