7ct4
From Proteopedia
(Difference between revisions)
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==Crystal structure of D-amino acid oxidase from Rasamsonia emersonii strain YA== | ==Crystal structure of D-amino acid oxidase from Rasamsonia emersonii strain YA== | ||
| - | <StructureSection load='7ct4' size='340' side='right'caption='[[7ct4]]' scene=''> | + | <StructureSection load='7ct4' size='340' side='right'caption='[[7ct4]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CT4 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7ct4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rasamsonia_emersonii Rasamsonia emersonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CT4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ct4 OCA], [https://pdbe.org/7ct4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ct4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ct4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ct4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A499UB99_TALEM A0A499UB99_TALEM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | D-Amino-acid oxidases (DAAOs) catalyze the oxidative deamination of neutral and basic D-amino acids. The DAAO from the thermophilic fungus Rasamsonia emersonii strain YA (ReDAAO) has a high thermal stability and a unique broad substrate specificity that includes the acidic D-amino acid D-Glu as well as various neutral and basic D-amino acids. In this study, ReDAAO was crystallized by the hanging-drop vapor-diffusion method and its crystal structure was determined at a resolution of 2.00 A. The crystal structure of the enzyme revealed that unlike other DAAOs, ReDAAO forms a homotetramer and contains an intramolecular disulfide bond (Cys230-Cys285), suggesting that this disulfide bond is involved in the higher thermal stability of ReDAAO. Moreover, the structure of the active site and its vicinity in ReDAAO indicates that Arg97, Lys99, Lys114 and Ser231 are candidates for recognizing the side chain of D-Glu. | ||
| + | |||
| + | X-ray structure analysis of a unique D-amino-acid oxidase from the thermophilic fungus Rasamsonia emersonii strain YA.,Shimekake Y, Hirato Y, Funabashi R, Okazaki S, Goto M, Furuichi T, Suzuki H, Kera Y, Takahashi S Acta Crystallogr F Struct Biol Commun. 2020 Nov 1;76(Pt 11):517-523. doi:, 10.1107/S2053230X20013333. Epub 2020 Oct 23. PMID:33135670<ref>PMID:33135670</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ct4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Rasamsonia emersonii]] | ||
[[Category: Funabashi R]] | [[Category: Funabashi R]] | ||
[[Category: Furuichi T]] | [[Category: Furuichi T]] | ||
Revision as of 16:15, 29 November 2023
Crystal structure of D-amino acid oxidase from Rasamsonia emersonii strain YA
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