7cv0
From Proteopedia
(Difference between revisions)
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==Crystal structure of B. halodurans NiaR in apo form== | ==Crystal structure of B. halodurans NiaR in apo form== | ||
| - | <StructureSection load='7cv0' size='340' side='right'caption='[[7cv0]]' scene=''> | + | <StructureSection load='7cv0' size='340' side='right'caption='[[7cv0]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CV0 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7cv0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_halodurans_C-125 Alkalihalobacillus halodurans C-125]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CV0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.998Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cv0 OCA], [https://pdbe.org/7cv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cv0 RCSB], [https://www.ebi.ac.uk/pdbsum/7cv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cv0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9KDJ7_HALH5 Q9KDJ7_HALH5] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The niacin-responsive repressor, NiaR, is transcriptional repressor of certain nicotinamide adenine dinucleotide (NAD) biosynthetic genes in response to an increase in niacin levels. NAD is a vital molecule involved in various cellular redox reactions as an electron donor or electron acceptor. The NiaR family is conserved broadly in the Bacillus/Clostridium group, as well as in the Fusobacteria and Thermotogales lineages. The NiaR structure consists of two domains: an N-terminal DNA-binding domain, and a C-terminal regulation domain containing a metal-binding site. In this paper, we report the crystal structures of apo and niacin-bound forms of NiaR from Bacillus halodurans (BhNiaR). The analysis of metal-binding and niacin-binding sites through the apo and niacin-bound structures is described. Each N- and C-terminal domain structure of BhNiaR is almost identical with NiaR from Thermotoga maritima, but the overall domain arrangement is quite different. A zinc ion is fully occupied in each subunit with well-conserved residues in the C-terminal domain. Niacin is also located at a hydrophobic pocket near the zinc ion in the C-terminal domain. | ||
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| + | Structural analysis and insight into effector binding of the niacin-responsive repressor NiaR from Bacillus halodurans.,Lee DW, Park YW, Lee MY, Jeong KH, Lee JY Sci Rep. 2020 Dec 3;10(1):21039. doi: 10.1038/s41598-020-78148-x. PMID:33273654<ref>PMID:33273654</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7cv0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Alkalihalobacillus halodurans C-125]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Jeong KH]] | [[Category: Jeong KH]] | ||
Current revision
Crystal structure of B. halodurans NiaR in apo form
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