7d27
From Proteopedia
(Difference between revisions)
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==Structure of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase== | ==Structure of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase== | ||
| - | <StructureSection load='7d27' size='340' side='right'caption='[[7d27]]' scene=''> | + | <StructureSection load='7d27' size='340' side='right'caption='[[7d27]], [[Resolution|resolution]] 2.48Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D27 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7d27]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D27 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d27 OCA], [https://pdbe.org/7d27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d27 RCSB], [https://www.ebi.ac.uk/pdbsum/7d27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d27 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.48Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d27 OCA], [https://pdbe.org/7d27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d27 RCSB], [https://www.ebi.ac.uk/pdbsum/7d27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d27 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | MurE ligase catalyzes the attachment of meso-diaminopimelic acid to the UDP-MurNAc-l -Ala-d -Glu using ATP and producing UDP-MurNAc-l -Ala-d -Glu-meso-A2 pm during bacterial cell wall biosynthesis. Owing to the critical role of this enzyme, MurE is considered an attractive target for antibacterial drugs. Despite extensive studies on MurE ligase, the structural dynamics of its conformational changes are still elusive. In this study, we present the substrate-free structure of MurE from Acinetobacter baumannii, which is an antibiotic-resistant superbacterium that has threatened global public health. The structure revealed that MurE has a wide-open conformation and undergoes wide-open, intermediately closed, and fully closed dynamic conformational transition. Unveiling structural dynamics of MurE will help to understand the working mechanism of this ligase and to design next-generation antibiotics targeting MurE. | ||
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| + | Wide-open conformation of UDP-MurNc-tripeptide ligase revealed by the substrate-free structure of MurE from Acinetobacter baumannii.,Jung KH, Kim YG, Kim CM, Ha HJ, Lee CS, Lee JH, Park HH FEBS Lett. 2021 Jan;595(2):275-283. doi: 10.1002/1873-3468.14007. Epub 2020 Dec, 3. PMID:33230844<ref>PMID:33230844</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7d27" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Mur ligase|Mur ligase]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Acinetobacter baumannii]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Jeong KH]] | [[Category: Jeong KH]] | ||
[[Category: Park HH]] | [[Category: Park HH]] | ||
Current revision
Structure of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase
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