7d2h
From Proteopedia
(Difference between revisions)
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==Tetrameric coiled-coil structure of liprin-alpha2_H2== | ==Tetrameric coiled-coil structure of liprin-alpha2_H2== | ||
| - | <StructureSection load='7d2h' size='340' side='right'caption='[[7d2h]]' scene=''> | + | <StructureSection load='7d2h' size='340' side='right'caption='[[7d2h]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7d2h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2H FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2h OCA], [https://pdbe.org/7d2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2h RCSB], [https://www.ebi.ac.uk/pdbsum/7d2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2h ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2h OCA], [https://pdbe.org/7d2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2h RCSB], [https://www.ebi.ac.uk/pdbsum/7d2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LIPA2_HUMAN LIPA2_HUMAN] Alters PTPRF cellular localization and induces PTPRF clustering. May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.<ref>PMID:9624153</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Synaptic scaffold proteins (e.g., liprin-alpha, ELKS, RIM, and RIM-BP) orchestrate ion channels, receptors, and enzymes at presynaptic terminals to form active zones for neurotransmitter release. The underlying mechanism of the active zone assembly remains elusive. Here, we report that liprin-alpha proteins have the potential to oligomerize through the N-terminal coiled-coil region. Our structural and biochemical characterizations reveal that a gain-of-function mutation promotes the self-assembly of the coiled coils in liprin-alpha2 by disrupting intramolecular interactions and promoting intermolecular interactions. By enabling multivalent interactions with ELKS proteins, the oligomerized coiled-coil region of liprin-alpha2 enhances the phase separation of the ELKS N-terminal segment. We further show that liprin-alpha2, by regulating the interplay between two phase separations of ELKS and RIM/RIM-BP, controls the protein distributions. These results imply that the complicated protein-protein interactions allow liprin-alpha to function with the active zone scaffolds and compartmentalize protein assemblies to achieve comprehensive functions in the active zone. | ||
| + | |||
| + | Oligomerized liprin-alpha promotes phase separation of ELKS for compartmentalization of presynaptic active zone proteins.,Liang M, Jin G, Xie X, Zhang W, Li K, Niu F, Yu C, Wei Z Cell Rep. 2021 Mar 23;34(12):108901. doi: 10.1016/j.celrep.2021.108901. PMID:33761347<ref>PMID:33761347</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7d2h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Liang M]] | [[Category: Liang M]] | ||
[[Category: Wei Z]] | [[Category: Wei Z]] | ||
Revision as of 16:24, 29 November 2023
Tetrameric coiled-coil structure of liprin-alpha2_H2
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