7d6c
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Crystal structure of CcmM N-terminal domain in complex with CcmN== |
| - | <StructureSection load='7d6c' size='340' side='right'caption='[[7d6c]]' scene=''> | + | <StructureSection load='7d6c' size='340' side='right'caption='[[7d6c]], [[Resolution|resolution]] 2.89Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7d6c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D6C FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d6c OCA], [https://pdbe.org/7d6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d6c RCSB], [https://www.ebi.ac.uk/pdbsum/7d6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d6c ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.89Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d6c OCA], [https://pdbe.org/7d6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d6c RCSB], [https://www.ebi.ac.uk/pdbsum/7d6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d6c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CCMM_SYNE7 CCMM_SYNE7] The presence of two potential DNA-binding regions suggests this protein may be a transcriptional regulator. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Carboxysome is an icosahedral self-assembled microcompartment that sequesters RuBisCO and carbonic anhydrases within a selectively permeable protein shell. The scaffolding proteins, CcmM, and CcmN were proposed to act as adaptors that crosslink the enzymatic core to shell facets. However, the details of interaction pattern remain unknown. Here we obtained a stable heterotrimeric complex of CcmM gamma-carbonic anhydrase domain (termed CcmM(NT) ) and CcmN, with a 1:2 stoichiometry, which interacts with the shell proteins CcmO and CcmL in vitro. The 2.9 A crystal structure of this heterotrimer revealed an asymmetric bundle composed of one CcmM(NT) and two CcmN subunits, all of which adopt a triangular left-handed beta-helical barrel structure. The central CcmN subunit packs against CcmM(NT) and another CcmN subunit via a wall-to-edge or wall-to-wall pattern, respectively. Together with previous findings, we propose CcmM(NT) -CcmN functions as an adaptor to facilitate the recruitment of shell proteins and the assembly of intact beta-carboxysome. | ||
| + | |||
| + | Complex structure reveals CcmM and CcmN form a heterotrimeric adaptor in beta-carboxysome.,Sun H, Cui N, Han SJ, Chen ZP, Xia LY, Chen Y, Jiang YL, Zhou CZ Protein Sci. 2021 Aug;30(8):1566-1576. doi: 10.1002/pro.4090. Epub 2021 May 8. PMID:33928692<ref>PMID:33928692</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7d6c" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Synechococcus elongatus PCC 7942 = FACHB-805]] |
| + | [[Category: Chen Y]] | ||
| + | [[Category: Chen ZP]] | ||
| + | [[Category: Cui N]] | ||
| + | [[Category: Han SJ]] | ||
| + | [[Category: Jiang YL]] | ||
| + | [[Category: Sun H]] | ||
| + | [[Category: Xia LY]] | ||
| + | [[Category: Zhou CZ]] | ||
Current revision
Crystal structure of CcmM N-terminal domain in complex with CcmN
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