7di3

From Proteopedia

(Difference between revisions)

Current revision

Cytochrome P450 (CYP105D18) W.T.

Structural highlights

7di3 is a 1 chain structure with sequence from Streptomyces laurentii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.69Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A160P685_STRLU

Publication Abstract from PubMed

The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study investigates the newly identified H2O2-mediated CYP105D18 from Streptomyces laurentii as the first bacterial CYP for N-oxidation. The catalytic efficiency of CYP105D18 for papaverine N-oxidation was 1.43 s(-1) microM (-1). The heme oxidation rate (k) was low (<0.3 min(-1)) in the presence of 200 mM H2O2. This high H2O2 tolerance capacity of CYP105D18 led to higher turnover prior to heme oxidation. Additionally, the high-resolution papaverine complexed structure and substrate-free structure of CYP105D18 were determined. Structural analysis and activity assay results revealed that CYP105D18 had a strong substrate preference for papaverine because of its bendable structure. These findings establish a basis for biotechnological applications of CYP105D18 in the pharmaceutical and medicinal industries.

Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation.,Pardhe BD, Do H, Jeong CS, Kim KH, Lee JH, Oh TJ IUCrJ. 2021 Jun 29;8(Pt 4):684-694. doi: 10.1107/S2052252521005522. eCollection, 2021 Jul 1. PMID:34258016^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pardhe BD, Do H, Jeong CS, Kim KH, Lee JH, Oh TJ. Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation. IUCrJ. 2021 Jun 29;8(Pt 4):684-694. doi: 10.1107/S2052252521005522. eCollection, 2021 Jul 1. PMID:34258016 doi:http://dx.doi.org/10.1107/S2052252521005522

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7di3"

Categories: Large Structures | Streptomyces laurentii | Do H | Lee JH

@@ Line 3: / Line 3: @@
 <StructureSection load='7di3' size='340' side='right'caption='[[7di3]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7di3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_31255 Atcc 31255]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DI3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7di3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_laurentii Streptomyces laurentii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DI3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SLA_5925 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39478 ATCC 31255])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7di3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7di3 OCA], [https://pdbe.org/7di3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7di3 RCSB], [https://www.ebi.ac.uk/pdbsum/7di3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7di3 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A160P685_STRLU A0A160P685_STRLU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 7di3" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Cytochrome P450 hydroxylase 3D structures|Cytochrome P450 hydroxylase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc 31255]]
 [[Category: Large Structures]]
-[[Category: Do, H]]
+[[Category: Streptomyces laurentii]]
-[[Category: Lee, J H]]
+[[Category: Do H]]
-[[Category: Chemical modification]]
+[[Category: Lee JH]]
-[[Category: Eukaryotic cytochrome p450]]
-[[Category: Heme oxidation]]
-[[Category: Papaverine n-oxide]]
-[[Category: Transferase]]

7di3

From Proteopedia

Current revision

Cytochrome P450 (CYP105D18) W.T.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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