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| | <StructureSection load='7e2r' size='340' side='right'caption='[[7e2r]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='7e2r' size='340' side='right'caption='[[7e2r]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7e2r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E2R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7e2r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E2R FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GUN4, At3g59400, F25L23_260 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e2r OCA], [https://pdbe.org/7e2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e2r RCSB], [https://www.ebi.ac.uk/pdbsum/7e2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e2r ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e2r OCA], [https://pdbe.org/7e2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e2r RCSB], [https://www.ebi.ac.uk/pdbsum/7e2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e2r ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/GUN4C_ARATH GUN4C_ARATH]] Regulates chlorophyll synthesis and plastid-to-nucleus signal transduction by binding both the product and the substrate of Mg-chelatase, an enzyme that produces magnesium-protoporphyrin IX (Mg-Proto). Activates also Mg-chelatase. Neither binds abscisic acid (ABA) nor is involved in ABA signaling.<ref>PMID:12574634</ref> <ref>PMID:23011401</ref>
| + | [https://www.uniprot.org/uniprot/GUN4C_ARATH GUN4C_ARATH] Regulates chlorophyll synthesis and plastid-to-nucleus signal transduction by binding both the product and the substrate of Mg-chelatase, an enzyme that produces magnesium-protoporphyrin IX (Mg-Proto). Activates also Mg-chelatase. Neither binds abscisic acid (ABA) nor is involved in ABA signaling.<ref>PMID:12574634</ref> <ref>PMID:23011401</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hu, J]] | + | [[Category: Hu J]] |
| - | [[Category: Liu, L]] | + | [[Category: Liu L]] |
| - | [[Category: Gun4]]
| + | |
| - | [[Category: Ligand binding protein]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
GUN4C_ARATH Regulates chlorophyll synthesis and plastid-to-nucleus signal transduction by binding both the product and the substrate of Mg-chelatase, an enzyme that produces magnesium-protoporphyrin IX (Mg-Proto). Activates also Mg-chelatase. Neither binds abscisic acid (ABA) nor is involved in ABA signaling.[1] [2]
Publication Abstract from PubMed
The chlorophyll biosynthesis regulator GENOMES UNCOUPLED 4 (GUN4) is conserved in nearly all oxygenic photosynthetic organisms. Recently, GUN4 has been found to be able to bind the linear tetrapyrroles (bilins) and stimulate the magnesium chelatase activity in the unicellular green alga Chlamydomonas reinhardtii. Here, we characterize GUN4 proteins from Arabidopsis thaliana and the cyanobacterium Synechocystis sp. PCC 6803 for their ability to bind bilins, and present the crystal structures of Synechocystis GUN4 in biliverdin-bound, phycocyanobilin-bound, and phytochromobilin-bound forms at the resolutions of 1.05, 1.10, and 1.70 A, respectively. These linear molecules adopt a cyclic-helical conformation, and bind more tightly than planar porphyrins to the tetrapyrrole-binding pocket of GUN4. Based on structural comparison, we propose a working model of GUN4 in regulation of tetrapyrrole biosynthetic pathway, and address the role of the bilin-bound GUN4 in retrograde signaling.
Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.,Hu JH, Chang JW, Xu T, Wang J, Wang X, Lin R, Duanmu D, Liu L Protein Sci. 2021 Aug 11. doi: 10.1002/pro.4164. PMID:34382282[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Larkin RM, Alonso JM, Ecker JR, Chory J. GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science. 2003 Feb 7;299(5608):902-6. doi: 10.1126/science.1079978. PMID:12574634 doi:http://dx.doi.org/10.1126/science.1079978
- ↑ Du SY, Zhang XF, Lu Z, Xin Q, Wu Z, Jiang T, Lu Y, Wang XF, Zhang DP. Roles of the different components of magnesium chelatase in abscisic acid signal transduction. Plant Mol Biol. 2012 Nov;80(4-5):519-37. doi: 10.1007/s11103-012-9965-3. Epub, 2012 Sep 27. PMID:23011401 doi:http://dx.doi.org/10.1007/s11103-012-9965-3
- ↑ Hu JH, Chang JW, Xu T, Wang J, Wang X, Lin R, Duanmu D, Liu L. Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4. Protein Sci. 2021 Aug 11. doi: 10.1002/pro.4164. PMID:34382282 doi:http://dx.doi.org/10.1002/pro.4164
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