1omh
From Proteopedia
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'''Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure.''' | '''Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure.''' | ||
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[[Category: Moncalian, G.]] | [[Category: Moncalian, G.]] | ||
[[Category: Perez-Luque, R.]] | [[Category: Perez-Luque, R.]] | ||
| - | [[Category: | + | [[Category: Bacterial conjugation]] |
| - | [[Category: | + | [[Category: Dna replication]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | [[Category: | + | [[Category: Relaxase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:01:50 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:01, 3 May 2008
Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure.
Overview
Relaxases are DNA strand transferases that catalyze the initial and final stages of DNA processing during conjugative cell-to-cell DNA transfer. Upon binding to the origin of transfer (oriT) DNA, relaxase TrwC melts the double helix. The three-dimensional structure of the relaxase domain of TrwC in complex with its cognate DNA at oriT shows a fold built on a two-layer alpha/beta sandwich, with a deep narrow cleft that houses the active site. The DNA includes one arm of an extruded cruciform, an essential feature for specific recognition. This arm is firmly embraced by the protein through a beta-ribbon positioned in the DNA major groove and a loop occupying the minor groove. It is followed by a single-stranded DNA segment that enters the active site, after a sharp U-turn forming a hydrophobic cage that traps the N-terminal methionine. Structural analysis combined with site-directed mutagenesis defines the architecture of the active site.
About this Structure
1OMH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC., Guasch A, Lucas M, Moncalian G, Cabezas M, Perez-Luque R, Gomis-Ruth FX, de la Cruz F, Coll M, Nat Struct Biol. 2003 Dec;10(12):1002-10. Epub 2003 Nov 16. PMID:14625590 Page seeded by OCA on Sat May 3 04:01:50 2008
