7esw
From Proteopedia
(Difference between revisions)
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==Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens== | ==Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens== | ||
| - | <StructureSection load='7esw' size='340' side='right'caption='[[7esw]]' scene=''> | + | <StructureSection load='7esw' size='340' side='right'caption='[[7esw]], [[Resolution|resolution]] 1.25Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ESW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ESW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7esw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_virens_Gv29-8 Trichoderma virens Gv29-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ESW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ESW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7esw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7esw OCA], [https://pdbe.org/7esw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7esw RCSB], [https://www.ebi.ac.uk/pdbsum/7esw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7esw ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7esw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7esw OCA], [https://pdbe.org/7esw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7esw RCSB], [https://www.ebi.ac.uk/pdbsum/7esw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7esw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SSCP_HYPVG SSCP_HYPVG] Stimulates salicylic acid signaling in host plant roots.<ref>PMID:34547313</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Trichoderma virens colonizes roots and develops a symbiotic relationship with plants where the fungal partner derives nutrients from plants and offers defence, in return. Tsp1, a small secreted cysteine-rich protein, was earlier found to be upregulated in co-cultivation of T. virens with maize roots. Tsp1 is well conserved in Ascomycota division of fungi, but none of its homologs have been studied yet. We have expressed and purified recombinant Tsp1, and resolved its structure to 1.25 A resolutions, from two crystal forms, using Se-SAD methods. The Tsp1 adopts a beta barrel fold and forms dimer in structure as well as in solution form. DALI based structure analysis revealed the structure similarity with two known fungal effector proteins: Alt a1 and PevD1. Structure and evolutionary analysis suggested that Tsp1 belongs to a novel effector protein family. Tsp1 acted as an inducer of salicylic acid mediated susceptibility in plants, rendering maize plants more susceptible to a necrotrophic pathogen Cochliobolus heterostrophus, as observed using plant defence assay and RT-qPCR analysis. | ||
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| + | Structure-function analysis reveals Trichoderma virens Tsp1 to be a novel fungal effector protein modulating plant defence.,Gupta GD, Bansal R, Mistry H, Pandey B, Mukherjee PK Int J Biol Macromol. 2021 Sep 20;191:267-276. doi:, 10.1016/j.ijbiomac.2021.09.085. PMID:34547313<ref>PMID:34547313</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7esw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Trichoderma virens Gv29-8]] | ||
[[Category: Gupta GD]] | [[Category: Gupta GD]] | ||
Current revision
Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens
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