7evf
From Proteopedia
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==Plant growth-promoting factor YxaL mutant from Bacillus velezensis - T175W/S213G/W215A== | ==Plant growth-promoting factor YxaL mutant from Bacillus velezensis - T175W/S213G/W215A== | ||
| - | <StructureSection load='7evf' size='340' side='right'caption='[[7evf]]' scene=''> | + | <StructureSection load='7evf' size='340' side='right'caption='[[7evf]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EVF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7evf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_velezensis_FZB42 Bacillus velezensis FZB42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EVF FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7evf OCA], [https://pdbe.org/7evf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7evf RCSB], [https://www.ebi.ac.uk/pdbsum/7evf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7evf ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7evf OCA], [https://pdbe.org/7evf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7evf RCSB], [https://www.ebi.ac.uk/pdbsum/7evf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7evf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A7ZAJ3_BACVZ A7ZAJ3_BACVZ] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The YxaL protein was isolated from the soil bacterium Bacillus velezensis and has been shown to promote the root growth of symbiotic plants. YxaL has further been suggested to act as an exogenous signaling protein to induce the growth and branching of plant roots. Amino acid sequence analysis predicted YxaL to exhibit an eight-bladed beta-propeller fold stabilized by six tryptophan-docking motifs and two modified motifs. Protein engineering to improve its structural stability is needed to increase the utility of YxaL as a plant growth-promoting factor. Here, the crystal structure of YxaL from B. velezensis was determined at 1.8 A resolution to explore its structural features for structure-based protein engineering. The structure showed the typical eight-bladed beta-propeller fold with structural variations in the third and fourth blades, which may decrease the stability of the beta-propeller fold. Engineered proteins targeting the modified motifs were subsequently created. Crystal structures of the engineered YxaL proteins showed that the typical tryptophan-docking interaction was restored in the third and fourth blades, with increased structural stability, resulting in improved root growth-promoting activity in Arabidopsis seeds. The work is an example of structure-based protein engineering to improve the structural stability of beta-propellor fold proteins. | ||
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| + | Structure of the plant growth-promoting factor YxaL from the rhizobacterium Bacillus velezensis and its application to protein engineering.,Kim J, Pham H, Baek Y, Jo I, Kim YH, Ha NC Acta Crystallogr D Struct Biol. 2022 Jan 1;78(Pt 1):104-112. doi:, 10.1107/S2059798321011724. Epub 2022 Jan 1. PMID:34981766<ref>PMID:34981766</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7evf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus velezensis FZB42]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ha N-C]] | [[Category: Ha N-C]] | ||
[[Category: Kim J]] | [[Category: Kim J]] | ||
Current revision
Plant growth-promoting factor YxaL mutant from Bacillus velezensis - T175W/S213G/W215A
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