7v1r
From Proteopedia
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==Leifsonia Alcohol Dehydrogenases LnADH== | ==Leifsonia Alcohol Dehydrogenases LnADH== | ||
| - | <StructureSection load='7v1r' size='340' side='right'caption='[[7v1r]]' scene=''> | + | <StructureSection load='7v1r' size='340' side='right'caption='[[7v1r]], [[Resolution|resolution]] 2.81Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V1R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7v1r]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Leifsonia_antarctica Leifsonia antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V1R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v1r OCA], [https://pdbe.org/7v1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v1r RCSB], [https://www.ebi.ac.uk/pdbsum/7v1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v1r ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v1r OCA], [https://pdbe.org/7v1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v1r RCSB], [https://www.ebi.ac.uk/pdbsum/7v1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v1r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/U2RWV7_LEIAQ U2RWV7_LEIAQ] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Leifsonia alcohol dehydrogenase (LnADH) is a promising biocatalyst for the synthesis of chiral alcohols. However, limitations of wild-type LnADH observed for practical application include low activity and poor stability. In this work, protein engineering was employed to improve its thermostability and catalytic efficiency by altering the subunit interfaces. Residues T100 and S148 were identified to be significant for thermostability and activity, and the melting temperature (DeltaTm ) and catalytic efficiency of the mutant T100R/S148I toward ketone substrates was improved by 18.7 degrees C and 1.8-5.5-fold. Solving the crystal structures of the wild-type enzyme and T100R/S148L revealed beneficial effects of mutations on stability and catalytic activity. The most robust mutant T100R/S148I is promising for industrial applications and can produce 200 g liter(-1) day(-1) chiral alcohols at 50 degrees C by only a 1 : 500 ratio of enzyme to substrate. | ||
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| + | Engineering Leifsonia Alcohol Dehydrogenase for Thermostability and Catalytic Efficiency by Enhancing Subunit Interactions.,Zhu L, Song Y, Chang C, Ma H, Yang L, Deng Z, Deng W, Qu X Chembiochem. 2021 Nov 16;22(22):3178-3183. doi: 10.1002/cbic.202100431. Epub 2021, Oct 1. PMID:34549865<ref>PMID:34549865</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7v1r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Leifsonia antarctica]] | ||
[[Category: Qu X]] | [[Category: Qu X]] | ||
[[Category: Song Y]] | [[Category: Song Y]] | ||
Current revision
Leifsonia Alcohol Dehydrogenases LnADH
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