7vmd

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==Crystal structure of a hydrolases Ple628 from marine microbial consortium==
==Crystal structure of a hydrolases Ple628 from marine microbial consortium==
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<StructureSection load='7vmd' size='340' side='right'caption='[[7vmd]]' scene=''>
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<StructureSection load='7vmd' size='340' side='right'caption='[[7vmd]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VMD FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7vmd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Unclassified_Marinobacter Unclassified Marinobacter]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VMD FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vmd OCA], [https://pdbe.org/7vmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vmd RCSB], [https://www.ebi.ac.uk/pdbsum/7vmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vmd ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vmd OCA], [https://pdbe.org/7vmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vmd RCSB], [https://www.ebi.ac.uk/pdbsum/7vmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vmd ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Polybutylene adipate terephthalate (PBAT) is a biodegradable alternative to polyethylene and can be broadly used in various applications. These polymers can be degraded by hydrolases of terrestrial and aquatic origin. In a previous study, we identified tandem PETase-like hydrolases (Ples) from the marine microbial consortium I1 that were highly expressed when a PBAT blend was supplied as the only carbon source. In this study, the tandem Ples, Ple628 and Ple629, were recombinantly expressed and characterized. Both enzymes are mesophilic and active on a wide range of oligomers. The activities of the Ples differed greatly when model substrates, PBAT-modified polymers or PET nanoparticles were supplied. Ple629 was always more active than Ple628. Crystal structures of Ple628 and Ple629 revealed a structural similarity to other PETases and can be classified as member of the PETases IIa subclass, alpha/beta hydrolase superfamily. Our results show that the predicted functions of Ple628 and Ple629 agree with the bioinformatic predictions, and these enzymes play a significant role in the plastic degradation by the consortium.
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Molecular and Biochemical Differences of the Tandem and Cold-Adapted PET Hydrolases Ple628 and Ple629, Isolated From a Marine Microbial Consortium.,Meyer Cifuentes IE, Wu P, Zhao Y, Liu W, Neumann-Schaal M, Pfaff L, Barys J, Li Z, Gao J, Han X, Bornscheuer UT, Wei R, Ozturk B Front Bioeng Biotechnol. 2022 Jul 21;10:930140. doi: 10.3389/fbioe.2022.930140., eCollection 2022. PMID:35935485<ref>PMID:35935485</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7vmd" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Unclassified Marinobacter]]
[[Category: Basak O]]
[[Category: Basak O]]
[[Category: Gao J]]
[[Category: Gao J]]

Revision as of 17:28, 29 November 2023

Crystal structure of a hydrolases Ple628 from marine microbial consortium

PDB ID 7vmd

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