7vnl
From Proteopedia
(Difference between revisions)
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==Sandercyanin mutant-F55A-Biliverdin complex== | ==Sandercyanin mutant-F55A-Biliverdin complex== | ||
| - | <StructureSection load='7vnl' size='340' side='right'caption='[[7vnl]]' scene=''> | + | <StructureSection load='7vnl' size='340' side='right'caption='[[7vnl]], [[Resolution|resolution]] 1.93Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VNL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vnl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sander_vitreus Sander vitreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VNL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vnl OCA], [https://pdbe.org/7vnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vnl RCSB], [https://www.ebi.ac.uk/pdbsum/7vnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vnl ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vnl OCA], [https://pdbe.org/7vnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vnl RCSB], [https://www.ebi.ac.uk/pdbsum/7vnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vnl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1D5B367_SANVI A0A1D5B367_SANVI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Biliverdin IXalpha (BV) binds to several prokaryotic and eukaryotic proteins. How nature exploits the versatility of BV's properties is not fully understood. Unlike free BV, the Sandercyanin fluorescent protein bound to BV (SFP-BV) shows enhanced red fluorescence (675 nm) on excitation in the UV region (380 nm). Site-directed mutagenesis showed that the BV complex of two SFP variants, F55A and E79A, resulted in the loss of red fluorescence. Crystal structures of the complexes of these proteins with BV show the absence of stacking interactions of the F55 phenyl ring with BV. BV changes from ZZZssa conformation in the wild-type to ZZZsss conformation in the variants. In the nonfluorescent mutants, the lowest excited state is destabilized, resulting in nonradiative decay. | ||
| + | |||
| + | Phenylalanine stacking enhances the red fluorescence of biliverdin IXalpha on UV excitation in sandercyanin fluorescent protein.,Yadav K, Ghosh S, Barak A, Schaefer W, Subramanian R FEBS Lett. 2022 Mar;596(6):796-805. doi: 10.1002/1873-3468.14281. Epub 2022 Jan, 17. PMID:35020202<ref>PMID:35020202</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vnl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Sander vitreus]] | ||
[[Category: Ghosh S]] | [[Category: Ghosh S]] | ||
[[Category: Subramanian R]] | [[Category: Subramanian R]] | ||
[[Category: Yadav K]] | [[Category: Yadav K]] | ||
Revision as of 17:28, 29 November 2023
Sandercyanin mutant-F55A-Biliverdin complex
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