7voc
From Proteopedia
(Difference between revisions)
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==The crystal structure of a Radical SAM Enzyme BlsE involved in the Biosynthesis of Blasticidin S== | ==The crystal structure of a Radical SAM Enzyme BlsE involved in the Biosynthesis of Blasticidin S== | ||
| - | <StructureSection load='7voc' size='340' side='right'caption='[[7voc]]' scene=''> | + | <StructureSection load='7voc' size='340' side='right'caption='[[7voc]], [[Resolution|resolution]] 2.62Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VOC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7voc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseochromogenes Streptomyces griseochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VOC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7voc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7voc OCA], [https://pdbe.org/7voc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7voc RCSB], [https://www.ebi.ac.uk/pdbsum/7voc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7voc ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6200542Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7QO:(2~{S},3~{S},4~{S},5~{R},6~{R})-6-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4,5-tris(oxidanyl)oxane-2-carboxylic+acid'>7QO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7voc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7voc OCA], [https://pdbe.org/7voc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7voc RCSB], [https://www.ebi.ac.uk/pdbsum/7voc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7voc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1B1AYF2_9ACTN A0A1B1AYF2_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The biosynthesis of blasticidin S has drawn attention due to the participation of the radical S-adenosyl methionine (SAM) enzyme BlsE. The original assignment of BlsE as a radical-mediated, redox-neutral decarboxylase is unusual because this reaction appears to serve no biosynthetic purpose and would need to be reversed by a subsequent carboxylation step. Furthermore, with the exception of BlsE, all other radical SAM decarboxylases reported to date are oxidative in nature. Careful analysis of the BlsE reaction, however, demonstrates that BlsE is not a decarboxylase but instead a lyase that catalyzes the dehydration of cytosylglucuronic acid (CGA) to form cytosyl-4'-keto-3'-deoxy-d-glucuronic acid, which can rapidly decarboxylate nonenzymatically in vitro. Analysis of substrate isotopologs, fluorinated analogues, as well as computational models based on X-ray crystal structures of the BlsE.SAM (2.09 A) and BlsE.SAM.CGA (2.62 A) complexes suggests that BlsE catalysis likely proceeds via direct elimination of water from the CGA C4' alpha-hydroxyalkyl radical as opposed to 1,2-migration of the C3'-hydroxyl prior to dehydration. Biosynthetic and mechanistic implications of the revised assignment of BlsE are discussed. | ||
| + | |||
| + | Radical S-Adenosyl Methionine Enzyme BlsE Catalyzes a Radical-Mediated 1,2-Diol Dehydration during the Biosynthesis of Blasticidin S.,Lee YH, Hou X, Chen R, Feng J, Liu X, Ruszczycky MW, Gao JM, Wang B, Zhou J, Liu HW J Am Chem Soc. 2022 Mar 16;144(10):4478-4486. doi: 10.1021/jacs.1c12010. Epub, 2022 Mar 3. PMID:35238201<ref>PMID:35238201</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7voc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces griseochromogenes]] | ||
[[Category: Hou XL]] | [[Category: Hou XL]] | ||
[[Category: Zhou JH]] | [[Category: Zhou JH]] | ||
Current revision
The crystal structure of a Radical SAM Enzyme BlsE involved in the Biosynthesis of Blasticidin S
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