7vpf
From Proteopedia
(Difference between revisions)
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==Crystal structure of a novel putative sugar isomerase from the psychrophilic bacterium Paenibacillus sp. R4== | ==Crystal structure of a novel putative sugar isomerase from the psychrophilic bacterium Paenibacillus sp. R4== | ||
| - | <StructureSection load='7vpf' size='340' side='right'caption='[[7vpf]]' scene=''> | + | <StructureSection load='7vpf' size='340' side='right'caption='[[7vpf]], [[Resolution|resolution]] 2.98Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VPF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vpf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_sp._FSL_H7-0331 Paenibacillus sp. FSL H7-0331]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VPF FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vpf OCA], [https://pdbe.org/7vpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vpf RCSB], [https://www.ebi.ac.uk/pdbsum/7vpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vpf ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.983Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vpf OCA], [https://pdbe.org/7vpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vpf RCSB], [https://www.ebi.ac.uk/pdbsum/7vpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vpf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1R1C9D1_9BACL A0A1R1C9D1_9BACL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sugar isomerases (SIs) catalyze the reversible conversion of aldoses to ketoses. A novel putative SI gene has been identified from the genome sequence information on the psychrophilic bacterium Paenibacillus sp. R4. Here, we report the crystal structure of the putative SI from Paenibacillus sp. R4 (PbSI) at 2.98 A resolution. It was found that the overall structure of PbSI adopts the triose-phosphate isomerase (TIM) barrel fold. PbSI was also identified to have two heterogeneous metal ions as its cofactors at the active site in the TIM barrel, one of which was confirmed as a Zn ion through X-ray anomalous scattering and inductively coupled plasma mass spectrometry analysis. Structural comparison with homologous SI proteins from mesophiles, hyperthermophiles, and a psychrophile revealed that key residues in the active site are well conserved and that dimeric PbSI is devoid of the extended C-terminal region, which tetrameric SIs commonly have. Our results provide novel structural information on the cold-adaptable SI, including information on the metal composition in the active site. | ||
| + | |||
| + | Crystal structure of a novel putative sugar isomerase from the psychrophilic bacterium Paenibacillus sp. R4.,Kwon S, Ha HJ, Kang YJ, Sung JH, Hwang J, Lee MJ, Lee JH, Park HH Biochem Biophys Res Commun. 2021 Dec 31;585:48-54. doi:, 10.1016/j.bbrc.2021.11.026. Epub 2021 Nov 11. PMID:34784551<ref>PMID:34784551</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vpf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Paenibacillus sp. FSL H7-0331]] | ||
[[Category: Kwon S]] | [[Category: Kwon S]] | ||
[[Category: Lee JH]] | [[Category: Lee JH]] | ||
[[Category: Park HH]] | [[Category: Park HH]] | ||
Current revision
Crystal structure of a novel putative sugar isomerase from the psychrophilic bacterium Paenibacillus sp. R4
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