This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1on9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1on9.jpg|left|200px]]
[[Image:1on9.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1on9 |SIZE=350|CAPTION= <scene name='initialview01'>1on9</scene>, resolution 2.00&Aring;
+
The line below this paragraph, containing "STRUCTURE_1on9", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MCA:METHYLMALONYL-COENZYME+A'>MCA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylmalonyl-CoA_carboxytransferase Methylmalonyl-CoA carboxytransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.1 2.1.3.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1on9| PDB=1on9 | SCENE= }}
-
|RELATEDENTRY=[[1on3|1ON3]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1on9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1on9 OCA], [http://www.ebi.ac.uk/pdbsum/1on9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1on9 RCSB]</span>
+
-
}}
+
'''Transcarboxylase 12S crystal structure: hexamer assembly and substrate binding to a multienzyme core (with hydrolyzed methylmalonyl-coenzyme a bound)'''
'''Transcarboxylase 12S crystal structure: hexamer assembly and substrate binding to a multienzyme core (with hydrolyzed methylmalonyl-coenzyme a bound)'''
Line 33: Line 30:
[[Category: Yee, V C.]]
[[Category: Yee, V C.]]
[[Category: Zheng, X.]]
[[Category: Zheng, X.]]
-
[[Category: carboxyl transferase]]
+
[[Category: Carboxyl transferase]]
-
[[Category: crystal structure]]
+
[[Category: Crystal structure]]
-
[[Category: domain duplication]]
+
[[Category: Domain duplication]]
-
[[Category: multienzyme complex]]
+
[[Category: Multienzyme complex]]
-
[[Category: transcarboxylase]]
+
[[Category: Transcarboxylase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:03:33 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:47:42 2008''
+

Revision as of 01:03, 3 May 2008

Image:1on9.jpg

Template:STRUCTURE 1on9

Transcarboxylase 12S crystal structure: hexamer assembly and substrate binding to a multienzyme core (with hydrolyzed methylmalonyl-coenzyme a bound)


Overview

Transcarboxylase from Propionibacterium shermanii is a 1.2 MDa multienzyme complex that couples two carboxylation reactions, transferring CO(2)(-) from methylmalonyl-CoA to pyruvate, yielding propionyl-CoA and oxaloacetate. The 1.9 A resolution crystal structure of the central 12S hexameric core, which catalyzes the first carboxylation reaction, has been solved bound to its substrate methylmalonyl-CoA. Overall, the structure reveals two stacked trimers related by 2-fold symmetry, and a domain duplication in the monomer. In the active site, the labile carboxylate group of methylmalonyl-CoA is stabilized by interaction with the N-termini of two alpha-helices. The 12S domains are structurally similar to the crotonase/isomerase superfamily, although only domain 1 of each 12S monomer binds ligand. The 12S reaction is similar to that of human propionyl-CoA carboxylase, whose beta-subunit has 50% sequence identity with 12S. A homology model of the propionyl-CoA carboxylase beta-subunit, based on this 12S crystal structure, provides new insight into the propionyl-CoA carboxylase mechanism, its oligomeric structure and the molecular basis of mutations responsible for enzyme deficiency in propionic acidemia.

About this Structure

1ON9 is a Single protein structure of sequence from Propionibacterium freudenreichii. Full crystallographic information is available from OCA.

Reference

Transcarboxylase 12S crystal structure: hexamer assembly and substrate binding to a multienzyme core., Hall PR, Wang YF, Rivera-Hainaj RE, Zheng X, Pustai-Carey M, Carey PR, Yee VC, EMBO J. 2003 May 15;22(10):2334-47. PMID:12743028 Page seeded by OCA on Sat May 3 04:03:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1on9"
Personal tools