7wwx
From Proteopedia
(Difference between revisions)
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==Crystal structure of Herbaspirillum huttiense L-arabinose 1-dehydrogenase (NAD bound form)== | ==Crystal structure of Herbaspirillum huttiense L-arabinose 1-dehydrogenase (NAD bound form)== | ||
| - | <StructureSection load='7wwx' size='340' side='right'caption='[[7wwx]]' scene=''> | + | <StructureSection load='7wwx' size='340' side='right'caption='[[7wwx]], [[Resolution|resolution]] 1.36Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WWX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7wwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Herbaspirillum_huttiense_subsp._putei_IAM_15032 Herbaspirillum huttiense subsp. putei IAM 15032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WWX FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wwx OCA], [https://pdbe.org/7wwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wwx RCSB], [https://www.ebi.ac.uk/pdbsum/7wwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wwx ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.36Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wwx OCA], [https://pdbe.org/7wwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wwx RCSB], [https://www.ebi.ac.uk/pdbsum/7wwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wwx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | l-Arabinose 1-dehydrogenase (AraDH) catalyzes the NAD(P)(+)-dependent oxidation of l-arabinose to L-arabinono-1,4-lactone in the non-phosphorylative l-arabinose pathway, and is classified into glucose-fructose oxidoreductase and short-chain dehydrogenase/reductase (SDR). We herein report the crystal structure of a SDR-type AraDH (from Herbaspirillum huttiense) for the first time. The interactions between Asp49 and the 2'- and 3'-hydroxyl groups of NAD(+) were consistent with strict specificity for NAD(+). In a binding model for the substrate, Ser155 and Tyr168, highly conserved in the SDR superfamily, interacted with the C1 and/or C2 hydroxyl(s) of l-arabinose, whereas interactions between Asp107, Arg109, and Gln206 and the C2 and/or C3 hydroxyl(s) were unique to AraDH. Trp200 significantly contributed to the selectivities of the C4 hydroxyl and C6 methyl of substrates. | ||
| + | |||
| + | Crystal structure of L-arabinose 1-dehydrogenase as a short-chain reductase/dehydrogenase protein.,Watanabe S, Yoshiwara K, Matsubara R, Watanabe Y Biochem Biophys Res Commun. 2022 Mar 8;604:14-21. doi:, 10.1016/j.bbrc.2022.03.028. PMID:35279441<ref>PMID:35279441</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7wwx" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Herbaspirillum huttiense subsp. putei IAM 15032]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Matsubara R]] | [[Category: Matsubara R]] | ||
Current revision
Crystal structure of Herbaspirillum huttiense L-arabinose 1-dehydrogenase (NAD bound form)
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