1ope

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[[Image:1ope.jpg|left|200px]]
[[Image:1ope.jpg|left|200px]]
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{{Structure
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|PDB= 1ope |SIZE=350|CAPTION= <scene name='initialview01'>1ope</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1ope", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacid_CoA-transferase 3-oxoacid CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.5 2.8.3.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= OXCT OR SCOT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa])
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|DOMAIN=
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{{STRUCTURE_1ope| PDB=1ope | SCENE= }}
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|RELATEDENTRY=[[1m3e|1M3E]], [[1ooy|1OOY]], [[1ooz|1OOZ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ope FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ope OCA], [http://www.ebi.ac.uk/pdbsum/1ope PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ope RCSB]</span>
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'''Deletion mutant of SUCCINYL-COA:3-KETOACID COA TRANSFERASE FROM PIG HEART'''
'''Deletion mutant of SUCCINYL-COA:3-KETOACID COA TRANSFERASE FROM PIG HEART'''
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[[Category: Swenson, L.]]
[[Category: Swenson, L.]]
[[Category: Wolodko, W T.]]
[[Category: Wolodko, W T.]]
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[[Category: alpha/beta protein]]
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[[Category: Alpha/beta protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:07:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:48:32 2008''
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Revision as of 01:07, 3 May 2008

Image:1ope.jpg

Template:STRUCTURE 1ope

Deletion mutant of SUCCINYL-COA:3-KETOACID COA TRANSFERASE FROM PIG HEART


Overview

Succinyl-CoA:3-ketoacid CoA transferase (SCOT; EC 2.8.3.5) activates the acetoacetate in ketone bodies by transferring the CoA group from succinyl-CoA to acetoacetate to produce acetoacetyl-CoA and succinate. In the reaction, a glutamate residue at the active site of the enzyme forms a thioester bond with CoA and in this form the enzyme is subject to autolytic fragmentation. The crystal structure of pig heart SCOT has been solved and refined to 1.7 A resolution in a new crystal form. The structure shows the active-site glutamate residue in a conformation poised for autolytic fragmentation, with its side chain accepting one hydrogen bond from Asn281 and another from its own amide N atom. However, the conformation of this glutamate side chain would have to change for the residues that are conserved in the CoA transferases (Gln99, Gly386 and Ala387) to participate in stabilizing the tetrahedral transition states of the catalytic mechanism. The structures of a deletion mutant in two different crystal forms were also solved.

About this Structure

1OPE is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Structure of the CoA transferase from pig heart to 1.7 A resolution., Coros AM, Swenson L, Wolodko WT, Fraser ME, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1717-25. Epub 2004, Sep 23. PMID:15388917 Page seeded by OCA on Sat May 3 04:07:32 2008

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