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| | <StructureSection load='5o22' size='340' side='right'caption='[[5o22]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='5o22' size='340' side='right'caption='[[5o22]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5o22]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O22 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5O22 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5o22]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O22 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C3R:Carolacton'>C3R</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C3R:Carolacton'>C3R</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5o22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o22 OCA], [http://pdbe.org/5o22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o22 RCSB], [http://www.ebi.ac.uk/pdbsum/5o22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o22 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o22 OCA], [https://pdbe.org/5o22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o22 RCSB], [https://www.ebi.ac.uk/pdbsum/5o22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o22 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FOLD_ECOLI FOLD_ECOLI]] Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.<ref>PMID:1748668</ref> | + | [https://www.uniprot.org/uniprot/FOLD_ECOLI FOLD_ECOLI] Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.<ref>PMID:1748668</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Koehnke, J]] | + | [[Category: Koehnke J]] |
| - | [[Category: Sikandar, A]] | + | [[Category: Sikandar A]] |
| - | [[Category: Carolacton]]
| + | |
| - | [[Category: Fold]]
| + | |
| - | [[Category: Inhibitor]]
| + | |
| - | [[Category: Natural product]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
FOLD_ECOLI Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.[1]
Publication Abstract from PubMed
The natural product carolacton is a macrolide keto-carboxylic acid produced by the myxobacterium Sorangium cellulosum, and was originally described as an antibacterial compound. Here we show that carolacton targets FolD, a key enzyme from the folate-dependent C1 metabolism. We characterize the interaction between bacterial FolD and carolacton biophysically, structurally and biochemically. Carolacton binds FolD with nanomolar affinity, and the crystal structure of the FolD-carolacton complex reveals the mode of binding. We show that the human FolD orthologs, MTHFD1 and MTHFD2, are also inhibited in the low nM range, and that micromolar concentrations of carolacton inhibit the growth of cancer cell lines. As mitochondrial MTHFD2 is known to be upregulated in cancer cells, it may be possible to use carolacton as an inhibitor tool compound to assess MTHFD2 as an anti-cancer target.
The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD.,Fu C, Sikandar A, Donner J, Zaburannyi N, Herrmann J, Reck M, Wagner-Dobler I, Koehnke J, Muller R Nat Commun. 2017 Nov 16;8(1):1529. doi: 10.1038/s41467-017-01671-5. PMID:29142318[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ D'Ari L, Rabinowitz JC. Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli. J Biol Chem. 1991 Dec 15;266(35):23953-8. PMID:1748668
- ↑ Fu C, Sikandar A, Donner J, Zaburannyi N, Herrmann J, Reck M, Wagner-Dobler I, Koehnke J, Muller R. The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD. Nat Commun. 2017 Nov 16;8(1):1529. doi: 10.1038/s41467-017-01671-5. PMID:29142318 doi:http://dx.doi.org/10.1038/s41467-017-01671-5
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