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| | ==Structure of the Cascade-I-Fv complex from Shewanella putrefaciens== | | ==Structure of the Cascade-I-Fv complex from Shewanella putrefaciens== |
| - | <StructureSection load='5o7h' size='340' side='right' caption='[[5o7h]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='5o7h' size='340' side='right'caption='[[5o7h]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5o7h]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Shepc Shepc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5o7h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_putrefaciens_CN-32 Shewanella putrefaciens CN-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O7H FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sputcn32_1823 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319224 SHEPC]), Sputcn32_1821 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319224 SHEPC]), Sputcn32_1822 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319224 SHEPC])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7h OCA], [http://pdbe.org/5o7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7h RCSB], [http://www.ebi.ac.uk/pdbsum/5o7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7h ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7h OCA], [https://pdbe.org/5o7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o7h RCSB], [https://www.ebi.ac.uk/pdbsum/5o7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7h ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A4Y6G3_SHEPC A4Y6G3_SHEPC] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5o7h" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5o7h" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[CRISPR subtype I-F|CRISPR subtype I-F]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Shepc]] | + | [[Category: Large Structures]] |
| - | [[Category: Altegoer, F]] | + | [[Category: Shewanella putrefaciens CN-32]] |
| - | [[Category: Bange, G]] | + | [[Category: Altegoer F]] |
| - | [[Category: Pausch, P]] | + | [[Category: Bange G]] |
| - | [[Category: Adaptive immunity]] | + | [[Category: Pausch P]] |
| - | [[Category: Antiviral protein]]
| + | |
| - | [[Category: Cascade]]
| + | |
| - | [[Category: Crispr]]
| + | |
| - | [[Category: Rna surveillance]]
| + | |
| Structural highlights
Function
A4Y6G3_SHEPC
Publication Abstract from PubMed
CRISPR-Cas systems are prokaryotic immune systems against invading nucleic acids. Type I CRISPR-Cas systems employ highly diverse, multi-subunit surveillance Cascade complexes that facilitate duplex formation between crRNA and complementary target DNA for R-loop formation, retention, and DNA degradation by the subsequently recruited nuclease Cas3. Typically, the large subunit recognizes bona fide targets through the PAM (protospacer adjacent motif), and the small subunit guides the non-target DNA strand. Here, we present the Apo- and target-DNA-bound structures of the I-Fv (type I-F variant) Cascade lacking the small and large subunits. Large and small subunits are functionally replaced by the 5' terminal crRNA cap Cas5fv and the backbone protein Cas7fv, respectively. Cas5fv facilitates PAM recognition from the DNA major groove site, in contrast to all other described type I systems. Comparison of the type I-Fv Cascade with an anti-CRISPR protein-bound I-F Cascade reveals that the type I-Fv structure differs substantially at known anti-CRISPR protein target sites and might therefore be resistant to viral Cascade interception.
Structural Variation of Type I-F CRISPR RNA Guided DNA Surveillance.,Pausch P, Muller-Esparza H, Gleditzsch D, Altegoer F, Randau L, Bange G Mol Cell. 2017 Aug 2. pii: S1097-2765(17)30494-X. doi:, 10.1016/j.molcel.2017.06.036. PMID:28781236[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pausch P, Muller-Esparza H, Gleditzsch D, Altegoer F, Randau L, Bange G. Structural Variation of Type I-F CRISPR RNA Guided DNA Surveillance. Mol Cell. 2017 Aug 2. pii: S1097-2765(17)30494-X. doi:, 10.1016/j.molcel.2017.06.036. PMID:28781236 doi:http://dx.doi.org/10.1016/j.molcel.2017.06.036
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