8kg9
From Proteopedia
(Difference between revisions)
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- | '''Unreleased structure''' | ||
- | + | ==Yeast replisome in state III== | |
+ | <StructureSection load='8kg9' size='340' side='right'caption='[[8kg9]], [[Resolution|resolution]] 4.52Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[8kg9]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8KG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8KG9 FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.52Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8kg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8kg9 OCA], [https://pdbe.org/8kg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8kg9 RCSB], [https://www.ebi.ac.uk/pdbsum/8kg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8kg9 ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/A0A6A5Q1S9_YEASX A0A6A5Q1S9_YEASX] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | The replisome that replicates the eukaryotic genome consists of at least three engines: the Cdc45-MCM-GINS (CMG) helicase that separates duplex DNA at the replication fork and two DNA polymerases, one on each strand, that replicate the unwound DNA. Here, we determined a series of cryo-electron microscopy structures of a yeast replisome comprising CMG, leading-strand polymerase Polepsilon and three accessory factors on a forked DNA. In these structures, Polepsilon engages or disengages with the motor domains of the CMG by occupying two alternative positions, which closely correlate with the rotational movement of the single-stranded DNA around the MCM pore. During this process, the polymerase remains stably coupled to the helicase using Psf1 as a hinge. This synergism is modulated by a concerted rearrangement of ATPase sites to drive DNA translocation. The Polepsilon-MCM coupling is not only required for CMG formation to initiate DNA replication but also facilitates the leading-strand DNA synthesis mediated by Polepsilon. Our study elucidates a mechanism intrinsic to the replisome that coordinates the activities of CMG and Polepsilon to negotiate any roadblocks, DNA damage, and epigenetic marks encountered during translocation along replication forks. | ||
- | + | Synergism between CMG helicase and leading strand DNA polymerase at replication fork.,Xu Z, Feng J, Yu D, Huo Y, Ma X, Lam WH, Liu Z, Li XD, Ishibashi T, Dang S, Zhai Y Nat Commun. 2023 Sep 20;14(1):5849. doi: 10.1038/s41467-023-41506-0. PMID:37730685<ref>PMID:37730685</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
- | [[Category: | + | <div class="pdbe-citations 8kg9" style="background-color:#fffaf0;"></div> |
- | [[Category: Dang | + | == References == |
- | [[Category: | + | <references/> |
- | [[Category: Xu | + | __TOC__ |
- | [[Category: Zhai | + | </StructureSection> |
+ | [[Category: Large Structures]] | ||
+ | [[Category: Saccharomyces cerevisiae]] | ||
+ | [[Category: Saccharomyces cerevisiae S288C]] | ||
+ | [[Category: Dang S]] | ||
+ | [[Category: Feng J]] | ||
+ | [[Category: Xu Z]] | ||
+ | [[Category: Yu D]] | ||
+ | [[Category: Zhai Y]] |
Current revision
Yeast replisome in state III
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