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| | ==NMR SOLUTION STRUCTURE OF APLYSIA ATTRACTIN== | | ==NMR SOLUTION STRUCTURE OF APLYSIA ATTRACTIN== |
| - | <StructureSection load='1t50' size='340' side='right'caption='[[1t50]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1t50' size='340' side='right'caption='[[1t50]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1t50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplca Aplca]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1nj7 1nj7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T50 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1t50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1nj7 1nj7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T50 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6500 APLCA])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t50 OCA], [https://pdbe.org/1t50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t50 RCSB], [https://www.ebi.ac.uk/pdbsum/1t50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t50 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t50 OCA], [https://pdbe.org/1t50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t50 RCSB], [https://www.ebi.ac.uk/pdbsum/1t50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t50 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ATT_APLCA ATT_APLCA]] Water-borne pheromone that attract the marine mollusk Aplysia into breeding aggregations and coordinate male and female reproductive behavior within the aggregation (By similarity).<ref>PMID:12917218</ref> <ref>PMID:15054104</ref>
| + | [https://www.uniprot.org/uniprot/ATT_APLCA ATT_APLCA] Water-borne pheromone that attract the marine mollusk Aplysia into breeding aggregations and coordinate male and female reproductive behavior within the aggregation (By similarity).<ref>PMID:12917218</ref> <ref>PMID:15054104</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aplca]] | + | [[Category: Aplysia californica]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Braun, W]] | + | [[Category: Braun W]] |
| - | [[Category: Nagle, G T]] | + | [[Category: Nagle GT]] |
| - | [[Category: Painter, S D]] | + | [[Category: Painter SD]] |
| - | [[Category: Rajaratnam, K]] | + | [[Category: Rajaratnam K]] |
| - | [[Category: Ravindranath, G]] | + | [[Category: Ravindranath G]] |
| - | [[Category: Schein, C H]] | + | [[Category: Schein CH]] |
| - | [[Category: Xu, Y]] | + | [[Category: Xu Y]] |
| - | [[Category: Aplysia attractin]]
| + | |
| - | [[Category: Attractin]]
| + | |
| - | [[Category: Mollusk]]
| + | |
| - | [[Category: Pheromone]]
| + | |
| Structural highlights
Function
ATT_APLCA Water-borne pheromone that attract the marine mollusk Aplysia into breeding aggregations and coordinate male and female reproductive behavior within the aggregation (By similarity).[1] [2]
Publication Abstract from PubMed
Water-borne protein pheromones are essential for coordination of reproductive activities in many marine organisms. In this paper, we describe the first structure of a pheromone protein from a marine organism, that of attractin (58 residues) from Aplysia californica. The NMR solution structure was determined from TOCSY, NOESY, and DQF-COSY measurements of recombinant attractin expressed in insect cells. The sequential resonance assignments were done with standard manual procedures. Approximately 90% of the 949 unambiguous NOESY cross-peaks were assigned automatically with simultaneous three-dimensional structure calculation using our NOAH/DIAMOD/FANTOM program suite. The final bundle of energy-refined structures is well-defined, with an average rmsd value to the mean structure of 0.72 +/- 0.12 A for backbone and 1.32 +/- 0.11 A for heavy atoms for amino acids 3-47. Attractin contains two antiparallel helices, made up of residues Ile9-Gln16 and I30-S36. The NMR distance constraints are consistent with the three disulfide bonds determined by mass spectroscopy (C4-C41, C13-C33, and C20-C26), where the first two could be directly determined from NOESY cross-peaks between CH beta protons of the corresponding cysteines. The second helix contains the (L/I)(29)IEECKTS(36) sequence conserved in attractins from five species of Aplysia that could interact with the receptor. The sequence and structure of this region are similar to those of the recognition helix of the Er-11 pheromone of the unicellular ciliate Euplotes raikovi, suggesting a possible common pathway for intercellular communication of these two distinct pheromone families.
NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica.,Garimella R, Xu Y, Schein CH, Rajarathnam K, Nagle GT, Painter SD, Braun W Biochemistry. 2003 Aug 26;42(33):9970-9. PMID:12924946[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Painter SD, Clough B, Black S, Nagle GT. Behavioral characterization of attractin, a water-borne peptide pheromone in the genus aplysia. Biol Bull. 2003 Aug;205(1):16-25. PMID:12917218
- ↑ Cummins SF, Nichols AE, Amare A, Hummon AB, Sweedler JV, Nagle GT. Characterization of Aplysia enticin and temptin, two novel water-borne protein pheromones that act in concert with attractin to stimulate mate attraction. J Biol Chem. 2004 Jun 11;279(24):25614-22. Epub 2004 Mar 30. PMID:15054104 doi:http://dx.doi.org/10.1074/jbc.M313585200
- ↑ Garimella R, Xu Y, Schein CH, Rajarathnam K, Nagle GT, Painter SD, Braun W. NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica. Biochemistry. 2003 Aug 26;42(33):9970-9. PMID:12924946 doi:http://dx.doi.org/10.1021/bi0274322
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