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| | <StructureSection load='5o99' size='340' side='right'caption='[[5o99]], [[Resolution|resolution]] 0.87Å' scene=''> | | <StructureSection load='5o99' size='340' side='right'caption='[[5o99]], [[Resolution|resolution]] 0.87Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5o99]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O99 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5O99 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5o99]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O99 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.871Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5o99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o99 OCA], [http://pdbe.org/5o99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o99 RCSB], [http://www.ebi.ac.uk/pdbsum/5o99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o99 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o99 OCA], [https://pdbe.org/5o99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o99 RCSB], [https://www.ebi.ac.uk/pdbsum/5o99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o99 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SHAN3_RAT SHAN3_RAT]] Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. | + | [https://www.uniprot.org/uniprot/SHAN3_RAT SHAN3_RAT] Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boeckers, T M]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Kursula, P]] | + | [[Category: Boeckers TM]] |
| - | [[Category: Myllykoski, M]] | + | [[Category: Kursula P]] |
| - | [[Category: Ponna, S K]] | + | [[Category: Myllykoski M]] |
| - | [[Category: Non-canonical binding site]] | + | [[Category: Ponna SK]] |
| - | [[Category: Postsynaptic density]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Scaffolding protein]]
| + | |
| - | [[Category: Sh3 domain]]
| + | |
| Structural highlights
Function
SHAN3_RAT Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Publication Abstract from PubMed
The Shank family comprises three large multi-domain proteins playing central roles as protein scaffolds in the neuronal postsynaptic density. The Shank proteins are closely linked to neuropsychiatric diseases, such as autism spectrum disorders. One characteristic domain in the Shank family is the SH3 domain, assumed to play a role in protein-protein interactions; however, no specific ligand binding to any Shank SH3 domain has been described. We solved the crystal structure of the SH3 domain from Shank3 at sub-atomic resolution. While the structure presents the canonical SH3 domain fold, the binding site for proline-rich peptides is not conserved. In line with this, no binding of Pro-rich sequences by the Shank3 SH3 domain was observed. Sequence comparisons indicate that all Shank isoforms have similarly lost the classical Pro-rich peptide binding site from the SH3 domain. Whether the corresponding site in the Shank SH3 domains has evolved to bind a non-poly-Pro target sequence is currently not known. Our work provides an intriguing example of the evolution of a well-characterized protein-protein interaction domain within the context of multi-domain protein scaffolds, allowing the conservation of structural features, but losing canonical functional sites. The data are further discussed in light of known mutations in the SH3 domain or its vicinity in the different Shank isoforms.
Structure of an unconventional SH3 domain from the postsynaptic density protein Shank3 at ultrahigh resolution.,Ponna SK, Myllykoski M, Boeckers TM, Kursula P Biochem Biophys Res Commun. 2017 Aug 26;490(3):806-812. doi:, 10.1016/j.bbrc.2017.06.121. Epub 2017 Jun 21. PMID:28647360[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ponna SK, Myllykoski M, Boeckers TM, Kursula P. Structure of an unconventional SH3 domain from the postsynaptic density protein Shank3 at ultrahigh resolution. Biochem Biophys Res Commun. 2017 Aug 26;490(3):806-812. doi:, 10.1016/j.bbrc.2017.06.121. Epub 2017 Jun 21. PMID:28647360 doi:http://dx.doi.org/10.1016/j.bbrc.2017.06.121
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