1os8

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[[Image:1os8.jpg|left|200px]]
[[Image:1os8.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1os8 |SIZE=350|CAPTION= <scene name='initialview01'>1os8</scene>, resolution 1.55&Aring;
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The line below this paragraph, containing "STRUCTURE_1os8", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= SPRT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1911 Streptomyces griseus])
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-->
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|DOMAIN=
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{{STRUCTURE_1os8| PDB=1os8 | SCENE= }}
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|RELATEDENTRY=[[1sgt|1SGT]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1os8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1os8 OCA], [http://www.ebi.ac.uk/pdbsum/1os8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1os8 RCSB]</span>
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}}
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'''RECOMBINANT STREPTOMYCES GRISEUS TRYPSIN'''
'''RECOMBINANT STREPTOMYCES GRISEUS TRYPSIN'''
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[[Category: Strynadka, N C.]]
[[Category: Strynadka, N C.]]
[[Category: Wong, S L.]]
[[Category: Wong, S L.]]
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[[Category: serine protease]]
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[[Category: Serine protease]]
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[[Category: trypsin]]
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[[Category: Trypsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:13:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:37 2008''
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Revision as of 01:13, 3 May 2008

Image:1os8.jpg

Template:STRUCTURE 1os8

RECOMBINANT STREPTOMYCES GRISEUS TRYPSIN


Overview

Streptomyces griseus trypsin (SGT) was chosen as a model scaffold for the development of serine proteases with enhanced substrate specificity. Recombinant SGT has been produced in a Bacillus subtilis expression system in a soluble active form and purified to homogeneity. The recombinant and native proteases have nearly identical enzymatic properties and structures. Four SGT mutants with alterations in the S1 substrate binding pocket (T190A, T190P, T190S, and T190V) were also expressed. The T190P mutant demonstrated the largest shift to a preference for Arg versus Lys in the P1 site. This was shown by a minor reduction in catalytic activity toward an Arg-containing substrate (k(cat) reduction of 25%). The crystal structures of the recombinant SGT and the T190P mutant in a complex with the inhibitor benzamidine were obtained at high resolution (approximately 1.9 A). The increase in P1 specificity, achieved with minimal effect on the catalytic efficiency, demonstrates that the T190P mutant is an ideal candidate for the design of additional substrate specificity engineered into the S2 to S4 binding pockets.

About this Structure

1OS8 is a Single protein structure of sequence from Streptomyces griseus. Full crystallographic information is available from OCA.

Reference

Engineering the primary substrate specificity of Streptomyces griseus trypsin., Page MJ, Wong SL, Hewitt J, Strynadka NC, MacGillivray RT, Biochemistry. 2003 Aug 5;42(30):9060-6. PMID:12885239 Page seeded by OCA on Sat May 3 04:13:15 2008

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