1ose

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[[Image:1ose.jpg|left|200px]]
[[Image:1ose.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ose |SIZE=350|CAPTION= <scene name='initialview01'>1ose</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1ose", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AS1:Active+Site,+Sugar+Binding+Site'>AS1</scene>, <scene name='pdbsite=AS2:Active+Site,+Sugar+Binding+Site'>AS2</scene>, <scene name='pdbsite=AS3:Active+Site,+Sugar+Binding+Site'>AS3</scene>, <scene name='pdbsite=AS4:Active+Site,+Sugar+Binding+Site'>AS4</scene>, <scene name='pdbsite=AS5:Active+Site,+Sugar+Binding+Site'>AS5</scene>, <scene name='pdbsite=CAL:Ca+Binding+Site'>CAL</scene> and <scene name='pdbsite=CLO:Chloride+Binding+Site.+There+Are+Six+Bound+Sugar+Rings.+...'>CLO</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1ose| PDB=1ose | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ose FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ose OCA], [http://www.ebi.ac.uk/pdbsum/1ose PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ose RCSB]</span>
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}}
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'''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE'''
'''PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE'''
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[[Category: Gilles, C.]]
[[Category: Gilles, C.]]
[[Category: Payan, F.]]
[[Category: Payan, F.]]
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[[Category: acarbose]]
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[[Category: Acarbose]]
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[[Category: alpha-amylase]]
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[[Category: Alpha-amylase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: hydrolase (o-glycosyl)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:13:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:46 2008''
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Revision as of 01:13, 3 May 2008

Image:1ose.jpg

Template:STRUCTURE 1ose

PORCINE PANCREATIC ALPHA-AMYLASE COMPLEXED WITH ACARBOSE


Overview

Two different crystal forms of pig pancreatic alpha-amylase isoenzyme II (PPAII), free and complexed to a carbohydrate inhibitor (acarbose), have been compared together and to previously reported structures of PPAI. A crystal form obtained at 4 degrees C, containing nearly 72% solvent, made it possible to obtain a new complex with acarbose, different from a previous one obtained at 20 degrees C [Qian, M., Buisson, G., Duee, E., Haser, H. & Payan, F. (1994) Biochemistry 33, 6284-6294]. In the present form, six contiguous subsites of the enzyme active site are occupied by the carbohydrate ligand; the structural data indicate that the binding site is capable of holding more than the five glucose units of the scheme proposed through kinetic studies. A monosaccharide ring bridging two protein molecules related by the crystal packing is located on the surface, at a distance of 2.0 nm from the reducing end of the inhibitor ligand; the symmetry-related glucose ring in the crystal lattice is found 1.5 nm away from the non-reducing end of the inhibitor ligand.

About this Structure

1OSE is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Crystal structure of pig pancreatic alpha-amylase isoenzyme II, in complex with the carbohydrate inhibitor acarbose., Gilles C, Astier JP, Marchis-Mouren G, Cambillau C, Payan F, Eur J Biochem. 1996 Jun 1;238(2):561-9. PMID:8681972 Page seeded by OCA on Sat May 3 04:13:41 2008

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