1ot2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1ot2.jpg|left|200px]]
[[Image:1ot2.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1ot2 |SIZE=350|CAPTION= <scene name='initialview01'>1ot2</scene>, resolution 2.10&Aring;
+
The line below this paragraph, containing "STRUCTURE_1ot2", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1ot2| PDB=1ot2 | SCENE= }}
-
|RELATEDENTRY=[[1cdg|1cdg]], [[1ot1|1OT1]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ot2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ot2 OCA], [http://www.ebi.ac.uk/pdbsum/1ot2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ot2 RCSB]</span>
+
-
}}
+
'''Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N'''
'''Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N'''
Line 28: Line 25:
[[Category: Dijkstra, B W.]]
[[Category: Dijkstra, B W.]]
[[Category: Rozeboom, H J.]]
[[Category: Rozeboom, H J.]]
-
[[Category: cyclodextrin]]
+
[[Category: Cyclodextrin]]
-
[[Category: glycosyl transferase]]
+
[[Category: Glycosyl transferase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:15:03 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:49:55 2008''
+

Revision as of 01:15, 3 May 2008

Image:1ot2.jpg

Template:STRUCTURE 1ot2

Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N


Overview

The alpha-amylase family is a large group of starch processing enzymes [Svensson, B. (1994) Plant Mol. Biol. 25, 141-157]. It is characterized by four short sequence motifs that contain the seven fully conserved amino acid residues in this family: two catalytic carboxylic acid residues and four substrate binding residues. The seventh conserved residue (Asp135) has no direct interactions with either substrates or products, but it is hydrogen-bonded to Arg227, which does bind the substrate in the catalytic site. Using cyclodextrin glycosyltransferase as an example, this paper provides for the first time definite biochemical and structural evidence that Asp135 is required for the proper conformation of several catalytic site residues and therefore for activity.

About this Structure

1OT2 is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

The fully conserved Asp residue in conserved sequence region I of the alpha-amylase family is crucial for the catalytic site architecture and activity., Leemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L, FEBS Lett. 2003 Apr 24;541(1-3):47-51. PMID:12706817 Page seeded by OCA on Sat May 3 04:15:03 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ot2"
Personal tools