1q4n

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spinqualitylabelsall models 1q4n, resolution 2.07Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structural studies of Phe256Trp of human salivary alpha-amylase: implications for the role of a conserved water molecule and its associated chain in enzyme activity

Overview

In the mechanism of hydrolysis of starch by alpha-amylases, a conserved, water molecule bridging two catalytic residues has been implicated. In, human salivary alpha-amylase (HSAmy), this water (W641), observed in many, alpha-amylase structures, is part of a chain of water molecules. To test, the hypothesis that W641 may be involved in the mechanism, Phe256 in the, close vicinity was mutated to a Trp residue. X-ray structure of F256W, complexed to 2-amino-2-(hydroxyethyl)-1,3-propanediol at 2.1A revealed, that the water chain is disrupted. In the F256W structure exhibits a, positional shift in His305, characteristic of alpha-amylase complex, structures. Kinetic analysis, in comparison with HSAmy, revealed that the, mutant exhibited a 70-fold decrease in the specific activity for starch, and significantly reduced k(cat) (20-fold) and K(m) (4-fold) for, maltoheptaoside. Collectively, these results suggest that W641 and the, chain of water molecules may be critical for the alpha-amylase activity.

About this Structure

1Q4N is a Single protein structure of sequence from Homo sapiens with CA, CL and TAM as ligands. Active as Alpha-amylase, with EC number 3.2.1.1 Full crystallographic information is available from OCA.

Reference

Structural studies of a Phe256Trp mutant of human salivary alpha-amylase: implications for the role of a conserved water molecule in enzyme activity., Ramasubbu N, Sundar K, Ragunath C, Rafi MM, Arch Biochem Biophys. 2004 Jan 1;421(1):115-24. PMID:14678792

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