1ou8
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ou8.jpg|left|200px]] | [[Image:1ou8.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ou8", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1ou8| PDB=1ou8 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''structure of an AAA+ protease delivery protein in complex with a peptide degradation tag''' | '''structure of an AAA+ protease delivery protein in complex with a peptide degradation tag''' | ||
| Line 30: | Line 27: | ||
[[Category: Sauer, R T.]] | [[Category: Sauer, R T.]] | ||
[[Category: Wah, D A.]] | [[Category: Wah, D A.]] | ||
| - | [[Category: | + | [[Category: Homodimer]] |
| - | [[Category: | + | [[Category: Peptide-binding pocket]] |
| - | [[Category: | + | [[Category: Protein-peptide complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:17:09 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:17, 3 May 2008
structure of an AAA+ protease delivery protein in complex with a peptide degradation tag
Overview
Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.
About this Structure
1OU8 is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
Reference
Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag., Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA, Mol Cell. 2003 Aug;12(2):365-72. PMID:14536076 Page seeded by OCA on Sat May 3 04:17:09 2008
