1oul
From Proteopedia
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'''Structure of the AAA+ protease delivery protein SspB''' | '''Structure of the AAA+ protease delivery protein SspB''' | ||
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[[Category: Sauer, R T.]] | [[Category: Sauer, R T.]] | ||
[[Category: Wah, D A.]] | [[Category: Wah, D A.]] | ||
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| - | [[Category: | + | [[Category: Ssra peptide binding protein]] |
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Revision as of 01:17, 3 May 2008
Structure of the AAA+ protease delivery protein SspB
Overview
Substrate selection by AAA+ ATPases that function to unfold proteins or alter protein conformation is often regulated by delivery or adaptor proteins. SspB is a protein dimer that binds to the ssrA degradation tag and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for degradation. Here, we describe the structure of the peptide binding domain of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution. The ssrA peptides are bound in well-defined clefts located at the extreme ends of the SspB homodimer. SspB contacts residues within the N-terminal and central regions of the 11 residue ssrA tag but leaves the C-terminal residues exposed and positioned to dock with ClpX. This structure, taken together with biochemical analysis of SspB, suggests mechanisms by which proteins like SspB escort substrates to AAA+ ATPases and enhance the specificity and affinity of target recognition.
About this Structure
1OUL is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
Reference
Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag., Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA, Mol Cell. 2003 Aug;12(2):365-72. PMID:14536076 Page seeded by OCA on Sat May 3 04:17:54 2008
