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1ovv
From Proteopedia
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'''CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)''' | '''CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVV OCA]. | |
==Reference== | ==Reference== | ||
Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876346 12876346] | Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876346 12876346] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Costanzo, L Di.]] | [[Category: Costanzo, L Di.]] | ||
[[Category: Geremia, S.]] | [[Category: Geremia, S.]] | ||
| - | [[Category: | + | [[Category: Alpha-helical bundle]] |
| - | [[Category: | + | [[Category: Protein design]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:20:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:20, 3 May 2008
CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)
Overview
Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an artificial protein with a four-helix bundle motif, di-Co(II)-DF1-L13A, have been collected using synchrotron radiation. The phase of form 1 has been assigned using the group and minimal non-isomorphic supergroup relation between the space group of the previously determined di-Mn(II)-DF1-L13G structure and the space group of this form. This unconventional method of solving the phase problem has also been tested with form 2 using a reverse relation. The structure of the latter form has been solved using the group and maximal non-isomorphic subgroup relation with the space group of form 2 of the analogous dimanganese protein. This application has shown that this phasing method can be used for solving the protein structures of polymorphic crystals as an alternative to the molecular-replacement method.
About this Structure
Full crystallographic information is available from OCA.
Reference
Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:12876346 Page seeded by OCA on Sat May 3 04:20:23 2008
