2bph

From Proteopedia

(Difference between revisions)

Current revision

STRUCTURE OF MURINE DECTIN-1

Structural highlights

2bph is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CLC7A_MOUSE Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The murine molecule dectin-1 (known as the beta-glucan receptor in humans) is an immune cell surface receptor implicated in the immunological defense against fungal pathogens. Sequence analysis has indicated that the dectin-1 extracellular domain is a C-type lectin-like domain, and functional studies have established that it binds fungal beta-glucans. We report several dectin-1 crystal structures, including a high-resolution structure and a 2.8 angstroms resolution structure in which a short soaked natural beta-glucan is trapped in the crystal lattice. In vitro characterization of dectin-1 in the presence of its natural ligand indicates higher-order complex formation between dectin-1 and beta-glucans. These combined structural and biophysical data considerably extend the current knowledge of dectin-1 structure and function, and suggest potential mechanisms of defense against fungal pathogens.

Structure of the fungal beta-glucan-binding immune receptor dectin-1: implications for function.,Brown J, O'Callaghan CA, Marshall AS, Gilbert RJ, Siebold C, Gordon S, Brown GD, Jones EY Protein Sci. 2007 Jun;16(6):1042-52. Epub 2007 May 1. PMID:17473009^[9]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ariizumi K, Shen GL, Shikano S, Xu S, Ritter R 3rd, Kumamoto T, Edelbaum D, Morita A, Bergstresser PR, Takashima A. Identification of a novel, dendritic cell-associated molecule, dectin-1, by subtractive cDNA cloning. J Biol Chem. 2000 Jun 30;275(26):20157-67. PMID:10779524 doi:http://dx.doi.org/10.1074/jbc.M909512199
↑ Brown GD, Gordon S. Immune recognition. A new receptor for beta-glucans. Nature. 2001 Sep 6;413(6851):36-7. PMID:11544516 doi:http://dx.doi.org/10.1038/35092620
↑ Gantner BN, Simmons RM, Canavera SJ, Akira S, Underhill DM. Collaborative induction of inflammatory responses by dectin-1 and Toll-like receptor 2. J Exp Med. 2003 May 5;197(9):1107-17. Epub 2003 Apr 28. PMID:12719479 doi:http://dx.doi.org/10.1084/jem.20021787
↑ Adachi Y, Ishii T, Ikeda Y, Hoshino A, Tamura H, Aketagawa J, Tanaka S, Ohno N. Characterization of beta-glucan recognition site on C-type lectin, dectin 1. Infect Immun. 2004 Jul;72(7):4159-71. PMID:15213161 doi:http://dx.doi.org/10.1128/IAI.72.7.4159-4171.2004
↑ Gantner BN, Simmons RM, Underhill DM. Dectin-1 mediates macrophage recognition of Candida albicans yeast but not filaments. EMBO J. 2005 Mar 23;24(6):1277-86. Epub 2005 Feb 24. PMID:15729357 doi:http://dx.doi.org/10.1038/sj.emboj.7600594
↑ Viriyakosol S, Fierer J, Brown GD, Kirkland TN. Innate immunity to the pathogenic fungus Coccidioides posadasii is dependent on Toll-like receptor 2 and Dectin-1. Infect Immun. 2005 Mar;73(3):1553-60. PMID:15731053 doi:http://dx.doi.org/73/3/1553
↑ Rogers NC, Slack EC, Edwards AD, Nolte MA, Schulz O, Schweighoffer E, Williams DL, Gordon S, Tybulewicz VL, Brown GD, Reis e Sousa C. Syk-dependent cytokine induction by Dectin-1 reveals a novel pattern recognition pathway for C type lectins. Immunity. 2005 Apr;22(4):507-17. PMID:15845454 doi:S1074-7613(05)00095-6
↑ Yadav M, Schorey JS. The beta-glucan receptor dectin-1 functions together with TLR2 to mediate macrophage activation by mycobacteria. Blood. 2006 Nov 1;108(9):3168-75. Epub 2006 Jul 6. PMID:16825490 doi:http://dx.doi.org/blood-2006-05-024406
↑ Brown J, O'Callaghan CA, Marshall AS, Gilbert RJ, Siebold C, Gordon S, Brown GD, Jones EY. Structure of the fungal beta-glucan-binding immune receptor dectin-1: implications for function. Protein Sci. 2007 Jun;16(6):1042-52. Epub 2007 May 1. PMID:17473009 doi:10.1110/ps.072791207

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bph"

@@ Line 3: / Line 3: @@
 <StructureSection load='2bph' size='340' side='right'caption='[[2bph]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bph]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BPH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bph]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BPH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bpd|2bpd]], [[2bpe|2bpe]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bph OCA], [https://pdbe.org/2bph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bph RCSB], [https://www.ebi.ac.uk/pdbsum/2bph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bph ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CLC7A_MOUSE CLC7A_MOUSE] Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.<ref>PMID:10779524</ref> <ref>PMID:11544516</ref> <ref>PMID:12719479</ref> <ref>PMID:15213161</ref> <ref>PMID:15729357</ref> <ref>PMID:15731053</ref> <ref>PMID:15845454</ref> <ref>PMID:16825490</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 34: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Brown, G D]]
+[[Category: Brown GD]]
-[[Category: Brown, J]]
+[[Category: Brown J]]
-[[Category: Callaghan, C A.O]]
+[[Category: Gilbert RJC]]
-[[Category: Gilbert, R J.C]]
+[[Category: Gordon S]]
-[[Category: Gordon, S]]
+[[Category: Jones EY]]
-[[Category: Jones, E Y]]
+[[Category: Marshall ASJ]]
-[[Category: Marshall, A S.J]]
+[[Category: O'Callaghan CA]]
-[[Category: Siebold, C]]
+[[Category: Siebold C]]
-[[Category: Beta-glucan]]
-[[Category: C-type lectin-like domain]]
-[[Category: Carbohydrate]]
-[[Category: Ctld]]
-[[Category: Dectin-1]]
-[[Category: Fungal recognition]]
-[[Category: Receptor]]

2bph

From Proteopedia

Current revision

STRUCTURE OF MURINE DECTIN-1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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