1q7d

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Revision as of 16:44, 12 November 2007

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spinqualitylabelsall models 1q7d, resolution 1.8Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structure of the integrin alpha2beta1 binding collagen peptide

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

We have determined the 1.8A crystal structure of a triple helical, integrin-binding collagen peptide (IBP) with sequence, (Gly-Pro-Hyp)(2)-Gly-Phe-Hyp-Gly-Glu-Arg-(Gly-Pro-Hyp)(3). The central, GFOGER hexapeptide is recognised specifically by the integrins, alpha2beta1, alpha1beta1, alpha10beta1 and alpha11beta1. These, integrin/collagen interactions are implicated in a number of key, physiological processes including cell adhesion, cell growth and, differentiation, and pathological states such as thrombosis and tumour, metastasis. Comparison of the IBP structure with the previously determined, structure of an identical collagen peptide in complex with the integrin, alpha2-I domain (IBP(c)) allows the first detailed examination of collagen, in a bound and an unbound state. The IBP structure shows a direct and a, water-mediated electrostatic interaction between Glu and Arg side-chains, from adjacent strands, but no intra-strand interactions. The interactions, between IBP Glu and Arg side-chains are disrupted upon integrin binding. A, comparison of IBP and IBP(c) main-chain conformation reveals the flexible, nature of the triple helix backbone in the imino-poor GFOGER region. This, flexibility could be important to the integrin-collagen interaction and, provides a possible explanation for the unique orientation of the three, GFOGER strands observed in the integrin-IBP(c) complex crystal structure.

Disease

Known diseases associated with this structure: Caffey disease OMIM:[120150], Dissection of cervical arteries OMIM:[120150], Ehlers-Danlos syndrome, type I OMIM:[120150], Ehlers-Danlos syndrome, type VII OMIM:[120150], OI type II OMIM:[120150], OI type III OMIM:[120150], OI type IV OMIM:[120150], OI/EDS combined syndrome OMIM:[120150], Osteogenesis imperfecta, type I OMIM:[120150], Osteoporosis OMIM:[120150]

About this Structure

1Q7D is a Single protein structure of sequence from [1] with ACE and NH2 as ligands. Full crystallographic information is available from OCA.

Reference

Structure of the integrin alpha2beta1-binding collagen peptide., Emsley J, Knight CG, Farndale RW, Barnes MJ, J Mol Biol. 2004 Jan 23;335(4):1019-28. PMID:14698296

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