1owf

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[[Image:1owf.gif|left|200px]]
[[Image:1owf.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1owf |SIZE=350|CAPTION= <scene name='initialview01'>1owf</scene>, resolution 1.95&Aring;
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The line below this paragraph, containing "STRUCTURE_1owf", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1owf| PDB=1owf | SCENE= }}
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|RELATEDENTRY=[[1ihf|1IHF]], [[1ouz|1OUZ]], [[1owg|1OWG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1owf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1owf OCA], [http://www.ebi.ac.uk/pdbsum/1owf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1owf RCSB]</span>
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}}
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'''Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site'''
'''Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site'''
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[[Category: Read, E K.]]
[[Category: Read, E K.]]
[[Category: Rice, P A.]]
[[Category: Rice, P A.]]
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[[Category: dna bending]]
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[[Category: Dna bending]]
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[[Category: ihf]]
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[[Category: Ihf]]
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[[Category: indirect readout]]
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[[Category: Indirect readout]]
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[[Category: minor groove]]
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[[Category: Minor groove]]
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[[Category: protein-dna recognition]]
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[[Category: Protein-dna recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:21:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:51:19 2008''
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Revision as of 01:21, 3 May 2008

Image:1owf.gif

Template:STRUCTURE 1owf

Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site


Overview

Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.

About this Structure

1OWF is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:12842466 Page seeded by OCA on Sat May 3 04:21:44 2008

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