2cc3

From Proteopedia

(Difference between revisions)

Current revision

Structure of Agrobacterium tumefaciens VirB8 protein

Structural highlights

2cc3 is a 2 chain structure with sequence from Agrobacterium tumefaciens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VIRB8_AGRFC VirB proteins are suggested to act at the bacterial surface and there play an important role in directing T-DNA transfer to plant cells.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Bacterial type IV secretion systems (T4SS) translocate DNA and/or proteins to recipient cells, thus providing a mechanism for conjugative transfer of genetic material and bacterial pathogenesis. Here we describe the first structure of a core component from the archetypal Agrobacterium tumefaciens T4SS: the 2.2-A resolution crystal structure of the VirB8 periplasmic domain (pVirB8(AT)). VirB8 forms a dimer in the crystal, and we identify residues likely important for stabilization of the dimer interface. Structural comparison of pVirB8(AT) with Brucella suis VirB8 confirms that the monomers have a similar fold. In addition, the pVirB8(AT) dimer superimposes very closely on the B. suis VirB8 dimer, supporting the proposal that dimer formation in the crystal reflects self-interactions that are biologically significant. The evolutionary conservation level for each residue was obtained from a data set of 84 VirB8 homologs and projected onto the protein structure to indicate conserved surface patches that likely contact other T4SS proteins.

Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites.,Bailey S, Ward D, Middleton R, Grossmann JG, Zambryski PC Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2582-7. Epub 2006 Feb 15. PMID:16481621^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bailey S, Ward D, Middleton R, Grossmann JG, Zambryski PC. Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites. Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2582-7. Epub 2006 Feb 15. PMID:16481621

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cc3"

@@ Line 3: / Line 3: @@
 <StructureSection load='2cc3' size='340' side='right'caption='[[2cc3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2cc3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_radiobacter"_(beijerinck_and_van_delden_1902)_bergey_et_al._1934 "achromobacter radiobacter" (beijerinck and van delden 1902) bergey et al. 1934]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CC3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2cc3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CC3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cc3 OCA], [https://pdbe.org/2cc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cc3 RCSB], [https://www.ebi.ac.uk/pdbsum/2cc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cc3 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/VIRB8_AGRFC VIRB8_AGRFC] VirB proteins are suggested to act at the bacterial surface and there play an important role in directing T-DNA transfer to plant cells.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 33: @@
 __TOC__
 </StructureSection>
+[[Category: Agrobacterium tumefaciens]]
 [[Category: Large Structures]]
-[[Category: Bailey, S]]
+[[Category: Bailey S]]
-[[Category: Grossmann, G]]
+[[Category: Grossmann G]]
-[[Category: Middleton, R]]
+[[Category: Middleton R]]
-[[Category: Ward, D]]
+[[Category: Ward D]]
-[[Category: Zambryski, P C]]
+[[Category: Zambryski PC]]
-[[Category: Agrobacterium]]
-[[Category: Consurf]]
-[[Category: Secretion system]]
-[[Category: Type iv secretion system]]
-[[Category: Virb8]]

2cc3

From Proteopedia

Current revision

Structure of Agrobacterium tumefaciens VirB8 protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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