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| | <StructureSection load='2cje' size='340' side='right'caption='[[2cje]], [[Resolution|resolution]] 2.34Å' scene=''> | | <StructureSection load='2cje' size='340' side='right'caption='[[2cje]], [[Resolution|resolution]] 2.34Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2cje]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2cje]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DUN:2-DEOXYURIDINE+5-ALPHA,BETA-IMIDO-DIPHOSPHATE'>DUN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.34Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2yay|2yay]], [[2yb0|2yb0]], [[2yaz|2yaz]], [[2cic|2cic]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DUN:2-DEOXYURIDINE+5-ALPHA,BETA-IMIDO-DIPHOSPHATE'>DUN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cje OCA], [https://pdbe.org/2cje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cje RCSB], [https://www.ebi.ac.uk/pdbsum/2cje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cje ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cje OCA], [https://pdbe.org/2cje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cje RCSB], [https://www.ebi.ac.uk/pdbsum/2cje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cje ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O15826_LEIMA O15826_LEIMA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leima]] | + | [[Category: Leishmania major]] |
| - | [[Category: DUTP diphosphatase]]
| + | [[Category: Fogg MJ]] |
| - | [[Category: Fogg, M J]] | + | [[Category: Gonzalez-Pacanowska D]] |
| - | [[Category: Gonzalez-Pacanowska, D]] | + | [[Category: Moroz OV]] |
| - | [[Category: Moroz, O V]] | + | [[Category: Wilson KS]] |
| - | [[Category: Wilson, K S]] | + | |
| - | [[Category: All-alpha]]
| + | |
| - | [[Category: Drug target]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Pathogen]]
| + | |
| Structural highlights
Function
O15826_LEIMA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Members of the Leishmania genus are the causative agents of the life-threatening disease leishmaniasis. New drugs are being sought due to increasing resistance and adverse side effects with current treatments. The knowledge that dUTPase is an essential enzyme and that the all alpha-helical dimeric kinetoplastid dUTPases have completely different structures compared with the trimeric beta-sheet type dUTPase possessed by most organisms, including humans, make the dimeric enzymes attractive drug targets. Here, we present crystal structures of the Leishmania major dUTPase in complex with substrate analogues, the product dUMP and a substrate fragment, and of the homologous Campylobacter jejuni dUTPase in complex with a triphosphate substrate analogue. The metal-binding properties of both enzymes are shown to be dependent upon the ligand identity, a previously unseen characteristic of this family. Furthermore, structures of the Leishmania enzyme in the presence of dUMP and deoxyuridine coupled with tryptophan fluorescence quenching indicate that occupation of the phosphate binding region is essential for induction of the closed conformation and hence for substrate binding. These findings will aid in the development of dUTPase inhibitors as potential new lead anti-trypanosomal compounds.
The Crystal Structure of the Leishmania major Deoxyuridine Triphosphate Nucleotidohydrolase in Complex with Nucleotide Analogues, dUMP, and Deoxyuridine.,Hemsworth GR, Moroz OV, Fogg MJ, Scott B, Bosch-Navarrete C, Gonzalez-Pacanowska D, Wilson KS J Biol Chem. 2011 May 6;286(18):16470-81. Epub 2011 Mar 15. PMID:21454646[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hemsworth GR, Moroz OV, Fogg MJ, Scott B, Bosch-Navarrete C, Gonzalez-Pacanowska D, Wilson KS. The Crystal Structure of the Leishmania major Deoxyuridine Triphosphate Nucleotidohydrolase in Complex with Nucleotide Analogues, dUMP, and Deoxyuridine. J Biol Chem. 2011 May 6;286(18):16470-81. Epub 2011 Mar 15. PMID:21454646 doi:10.1074/jbc.M111.224873
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