1oxe

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[[Image:1oxe.jpg|left|200px]]
[[Image:1oxe.jpg|left|200px]]
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{{Structure
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|PDB= 1oxe |SIZE=350|CAPTION= <scene name='initialview01'>1oxe</scene>, resolution 1.15&Aring;
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The line below this paragraph, containing "STRUCTURE_1oxe", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=NYC:(5Z)-5-(1H-INDOL-3-YLMETHYLENE)-4H-IMIDAZOL-4-ONE'>NYC</scene>
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{{STRUCTURE_1oxe| PDB=1oxe | SCENE= }}
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|RELATEDENTRY=[[1emb|1EMB]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxe OCA], [http://www.ebi.ac.uk/pdbsum/1oxe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oxe RCSB]</span>
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'''Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins'''
'''Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins'''
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[[Category: Wiegand, G.]]
[[Category: Wiegand, G.]]
[[Category: Zumbusch, A.]]
[[Category: Zumbusch, A.]]
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[[Category: amino acid incorporation]]
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[[Category: Amino acid incorporation]]
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[[Category: chromophore]]
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[[Category: Chromophore]]
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[[Category: genetic code]]
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[[Category: Genetic code]]
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[[Category: green fluorescent protein]]
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[[Category: Green fluorescent protein]]
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[[Category: tryptophan]]
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[[Category: Tryptophan]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:23:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:51:50 2008''
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Revision as of 01:23, 3 May 2008

Image:1oxe.jpg


PDB ID 1oxe

Drag the structure with the mouse to rotate
1oxe, resolution 1.15Å ()
Non-Standard Residues:
Related: 1emb
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins


Overview

Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.

About this Structure

1OXE is a Single protein structure of sequence from Cfp marker plasmid pwm1009. Full crystallographic information is available from OCA.

Reference

Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins., Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N, J Mol Biol. 2003 May 16;328(5):1071-81. PMID:12729742 Page seeded by OCA on Sat May 3 04:23:38 2008

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