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| | <StructureSection load='2v32' size='340' side='right'caption='[[2v32]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='2v32' size='340' side='right'caption='[[2v32]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2v32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arema Arema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V32 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2v32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arenicola_marina Arenicola marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V32 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2v2g|2v2g]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v32 OCA], [https://pdbe.org/2v32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v32 RCSB], [https://www.ebi.ac.uk/pdbsum/2v32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v32 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v32 OCA], [https://pdbe.org/2v32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v32 RCSB], [https://www.ebi.ac.uk/pdbsum/2v32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v32 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q1AN22_AREMA Q1AN22_AREMA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arema]] | |
| - | [[Category: Large Structures]] | |
| - | [[Category: Peroxiredoxin]] | |
| - | [[Category: Declercq, J P]] | |
| - | [[Category: Smeets, A]] | |
| - | [[Category: Antioxidant enzyme]] | |
| | [[Category: Arenicola marina]] | | [[Category: Arenicola marina]] |
| - | [[Category: Oxidoreductase]] | + | [[Category: Large Structures]] |
| | + | [[Category: Declercq JP]] |
| | + | [[Category: Smeets A]] |
| Structural highlights
Function
Q1AN22_AREMA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The peroxiredoxins (PRDXs) define a superfamily of thiol-dependent peroxidases able to reduce hydrogen peroxide, alkyl hydroperoxides, and peroxynitrite. Besides their cytoprotective antioxidant function, PRDXs have been implicated in redox signaling and chaperone activity, the latter depending on the formation of decameric high-molecular-weight structures. PRDXs have been mechanistically divided into three major subfamilies, namely typical 2-Cys, atypical 2-Cys, and 1-Cys PRDXs, based on the number and position of cysteines involved in the catalysis. We report the structure of the C45S mutant of annelid worm Arenicola marina PRDX6 in three different crystal forms determined at 1.6, 2.0, and 2.4 A resolution. Although A. marina PRDX6 was cloned during the search of annelid homologs of mammalian 1-Cys PRDX6s, the crystal structures support its assignment to the mechanistically typical 2-Cys PRDX subfamily. The protein is composed of two distinct domains: a C-terminal domain and an N-terminal domain exhibiting a thioredoxin fold. The subunits are associated in dimers compatible with the formation of intersubunit disulfide bonds between the peroxidatic and the resolving cysteine residues in the wild-type enzyme. The packing of two crystal forms is very similar, with pairs of dimers associated as tetramers. The toroid-shaped decamers formed by dimer association and observed in most typical 2-Cys PRDXs is not present. Thus, A. marina PRDX6 presents structural features of typical 2-Cys PRDXs without any formation of toroid-shaped decamers, suggesting that it should function more like a cytoprotective antioxidant enzyme or a modulator of peroxide-dependent cell signaling rather than a molecular chaperone.
The crystal structure of the C45S mutant of annelid Arenicola marina peroxiredoxin 6 supports its assignment to the mechanistically typical 2-Cys subfamily without any formation of toroid-shaped decamers.,Smeets A, Loumaye E, Clippe A, Rees JF, Knoops B, Declercq JP Protein Sci. 2008 Apr;17(4):700-10. PMID:18359859[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Smeets A, Loumaye E, Clippe A, Rees JF, Knoops B, Declercq JP. The crystal structure of the C45S mutant of annelid Arenicola marina peroxiredoxin 6 supports its assignment to the mechanistically typical 2-Cys subfamily without any formation of toroid-shaped decamers. Protein Sci. 2008 Apr;17(4):700-10. PMID:18359859 doi:17/4/700
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