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| | <StructureSection load='2vdx' size='340' side='right'caption='[[2vdx]], [[Resolution|resolution]] 1.84Å' scene=''> | | <StructureSection load='2vdx' size='340' side='right'caption='[[2vdx]], [[Resolution|resolution]] 1.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VDX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VDX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vdy|2vdy]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vdx OCA], [https://pdbe.org/2vdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vdx RCSB], [https://www.ebi.ac.uk/pdbsum/2vdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vdx ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vdx OCA], [https://pdbe.org/2vdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vdx RCSB], [https://www.ebi.ac.uk/pdbsum/2vdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vdx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[https://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN]] Corticosteroid-binding globulin deficiency. The disease is caused by mutations affecting the gene represented in this entry.
| + | [https://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN] Corticosteroid-binding globulin deficiency. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN]] Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.<ref>PMID:18513745</ref>
| + | [https://www.uniprot.org/uniprot/CBG_HUMAN CBG_HUMAN] Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.<ref>PMID:18513745</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Read, R J]] | + | [[Category: Read RJ]] |
| - | [[Category: Wei, Z]] | + | [[Category: Wei Z]] |
| - | [[Category: Zhou, A]] | + | [[Category: Zhou A]] |
| - | [[Category: Cbg]]
| + | |
| - | [[Category: Cleaved]]
| + | |
| - | [[Category: Cortisol]]
| + | |
| - | [[Category: Cortisol binding globulin]]
| + | |
| - | [[Category: Disease mutation]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Lipid-binding]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Serpin]]
| + | |
| - | [[Category: Steroid-binding]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Disease
CBG_HUMAN Corticosteroid-binding globulin deficiency. The disease is caused by mutations affecting the gene represented in this entry.
Function
CBG_HUMAN Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Corticosteroids are transported in the blood by a serpin, corticosteroid-binding globulin (CBG), and their normally equilibrated release can be further triggered by the cleavage of the reactive loop of CBG. We report here the crystal structures of cleaved human CBG (cCBG) at 1.8-A resolution and its complex with cortisol at 2.3-A resolution. As expected, on cleavage, CBG undergoes the irreversible S-to-R serpin transition, with the cleaved reactive loops being fully incorporated into the central beta-sheet. A connecting loop of helix D, which is in a helix-like conformation in native CBG, unwinds and grossly perturbs the hormone binding site following beta-sheet expansion in the cCBG structure but shifts away from the binding site by more than 8 A following the binding of cortisol. Unexpectedly, on cortisol binding, the hormone binding site of cCBG adopts a configuration almost identical with that of the native conformer. We conclude that CBG has adapted an allosteric mechanism of the serpins to allow equilibrated release of the hormones by a flip-flop movement of the intact reactive loop into and out of the beta-sheet. The change in the hormone binding affinity results from a change in the flexibility or plasticity of the connecting loop, which modulates the configuration of the binding site.
The S-to-R transition of corticosteroid-binding globulin and the mechanism of hormone release.,Zhou A, Wei Z, Stanley PL, Read RJ, Stein PE, Carrell RW J Mol Biol. 2008 Jun 27;380(1):244-51. Epub 2008 May 14. PMID:18513745[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhou A, Wei Z, Stanley PL, Read RJ, Stein PE, Carrell RW. The S-to-R transition of corticosteroid-binding globulin and the mechanism of hormone release. J Mol Biol. 2008 Jun 27;380(1):244-51. Epub 2008 May 14. PMID:18513745 doi:10.1016/j.jmb.2008.05.012
- ↑ Zhou A, Wei Z, Stanley PL, Read RJ, Stein PE, Carrell RW. The S-to-R transition of corticosteroid-binding globulin and the mechanism of hormone release. J Mol Biol. 2008 Jun 27;380(1):244-51. Epub 2008 May 14. PMID:18513745 doi:10.1016/j.jmb.2008.05.012
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