5oej

From Proteopedia

(Difference between revisions)

Current revision

Structure of Tra1 subunit within the chromatin modifying complex SAGA

5oej, resolution 5.70Å

Structural highlights

5oej is a 1 chain structure with sequence from Komagataella pastoris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 5.7Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C4QYV4_KOMPG

Publication Abstract from PubMed

The transcription co-activator complex SAGA is recruited to gene promoters by sequence-specific transcriptional activators and by chromatin modifications to promote pre-initiation complex formation. The yeast Tra1 subunit is the major target of acidic activators such as Gal4, VP16, or Gcn4 but little is known about its structural organization. The 430 kDa Tra1 subunit and its human homolog the transformation/transcription domain-associated protein TRRAP are members of the phosphatidyl 3-kinase-related kinase (PIKK) family. Here, we present the cryo-EM structure of the entire SAGA complex where the major target of activator binding, the 430 kDa Tra1 protein, is resolved with an average resolution of 5.7 A. The high content of alpha-helices in Tra1 enabled tracing of the majority of its main chain. Our results highlight the integration of Tra1 within the major epigenetic regulator SAGA.

Structure of the transcription activator target Tra1 within the chromatin modifying complex SAGA.,Sharov G, Voltz K, Durand A, Kolesnikova O, Papai G, Myasnikov AG, Dejaegere A, Shem AB, Schultz P Nat Commun. 2017 Nov 16;8(1):1556. doi: 10.1038/s41467-017-01564-7. PMID:29146944^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sharov G, Voltz K, Durand A, Kolesnikova O, Papai G, Myasnikov AG, Dejaegere A, Shem AB, Schultz P. Structure of the transcription activator target Tra1 within the chromatin modifying complex SAGA. Nat Commun. 2017 Nov 16;8(1):1556. doi: 10.1038/s41467-017-01564-7. PMID:29146944 doi:http://dx.doi.org/10.1038/s41467-017-01564-7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5oej"

@@ Line 3: / Line 3: @@
 <SX load='5oej' size='340' side='right' viewer='molstar' caption='[[5oej]], [[Resolution|resolution]] 5.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5oej]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OEJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OEJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5oej]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OEJ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5oej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oej OCA], [http://pdbe.org/5oej PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oej RCSB], [http://www.ebi.ac.uk/pdbsum/5oej PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oej ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oej OCA], [https://pdbe.org/5oej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oej RCSB], [https://www.ebi.ac.uk/pdbsum/5oej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oej ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C4QYV4_KOMPG C4QYV4_KOMPG]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 24: @@
 [[Category: Komagataella pastoris]]
 [[Category: Large Structures]]
-[[Category: Ben-Shem, A]]
+[[Category: Ben-Shem A]]
-[[Category: Dejaegere, A]]
+[[Category: Dejaegere A]]
-[[Category: Durand, A]]
+[[Category: Durand A]]
-[[Category: Kolesnikova, O]]
+[[Category: Kolesnikova O]]
-[[Category: Myasnikov, A G]]
+[[Category: Myasnikov AG]]
-[[Category: Papai, G]]
+[[Category: Papai G]]
-[[Category: Schultz, P]]
+[[Category: Schultz P]]
-[[Category: Sharov, G]]
+[[Category: Sharov G]]
-[[Category: Voltz, K]]
+[[Category: Voltz K]]
-[[Category: Activator target]]
-[[Category: Saga]]
-[[Category: Tra1]]
-[[Category: Transcription]]
-[[Category: Yeast]]

5oej

From Proteopedia

Current revision

Structure of Tra1 subunit within the chromatin modifying complex SAGA

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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