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1p0u
From Proteopedia
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[[Image:1p0u.gif|left|200px]] | [[Image:1p0u.gif|left|200px]] | ||
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'''Sheared G/C Base Pair''' | '''Sheared G/C Base Pair''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0U OCA]. | |
==Reference== | ==Reference== | ||
Sheared-type G(anti).C(syn) base-pair: a unique d(GXC) loop closure motif., Chin KH, Chou SH, J Mol Biol. 2003 May 30;329(2):351-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12758081 12758081] | Sheared-type G(anti).C(syn) base-pair: a unique d(GXC) loop closure motif., Chin KH, Chou SH, J Mol Biol. 2003 May 30;329(2):351-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12758081 12758081] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Chin, K H.]] | [[Category: Chin, K H.]] | ||
[[Category: Chou, S H.]] | [[Category: Chou, S H.]] | ||
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Sheared gc base pair]] |
| - | [[Category: | + | [[Category: Single-residue loop]] |
| - | [[Category: | + | [[Category: Syn cytidine]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:32:25 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:32, 3 May 2008
Sheared G/C Base Pair
Overview
Stable DNA loop structures closed by a novel G.C base-pair have been determined for the single-residue d(GXC) loops (X=A, T, G or C) in low-salt solution by high-resolution nuclear magnetic resonance (NMR) techniques. The closing G.C base-pair in these loops is not of the canonical Watson-Crick type, but adopts instead a unique sheared-type (trans Watson-Crick/sugar-edge) pairing, like those occurring in the sheared mismatched G.A or A.C base-pair, to draw the two opposite strands together. The cytidine residue in the closing base-pair is transformed into the rare syn domain to form two H-bonds with the guanine base and to prevent the steric clash between the G 2NH(2) and the C H-5 protons. Besides, the sugar pucker of the syn cytidine is still located in the regular C2'-endo domain, unlike the C3'-endo domain adopted for the pyrimidines of the out-of-alternation left-handed Z-DNA structure. The facile formation of the compact d(GXC) loops closed by a unique sheared-type G(anti).C(syn) base-pair demonstrates the great potential of the single-stranded d(GXC) triplet repeats to fold into stable hairpins.
About this Structure
Full crystallographic information is available from OCA.
Reference
Sheared-type G(anti).C(syn) base-pair: a unique d(GXC) loop closure motif., Chin KH, Chou SH, J Mol Biol. 2003 May 30;329(2):351-61. PMID:12758081 Page seeded by OCA on Sat May 3 04:32:25 2008
