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| | ==Endonuclease NucB== | | ==Endonuclease NucB== |
| - | <StructureSection load='5omt' size='340' side='right' caption='[[5omt]], [[Resolution|resolution]] 1.35Å' scene=''> | + | <StructureSection load='5omt' size='340' side='right'caption='[[5omt]], [[Resolution|resolution]] 1.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5omt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OMT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5omt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OMT FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nucB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1402 "Clostridium licheniforme" Weigmann 1898])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5omt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5omt OCA], [http://pdbe.org/5omt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5omt RCSB], [http://www.ebi.ac.uk/pdbsum/5omt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5omt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5omt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5omt OCA], [https://pdbe.org/5omt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5omt RCSB], [https://www.ebi.ac.uk/pdbsum/5omt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5omt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F1BV52_BACLI F1BV52_BACLI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Clostridium licheniforme weigmann 1898]]
| + | [[Category: Bacillus licheniformis]] |
| - | [[Category: Basle, A]]
| + | [[Category: Large Structures]] |
| - | [[Category: Lewis, R J]]
| + | [[Category: Basle A]] |
| - | [[Category: Bacillus subtili]] | + | [[Category: Lewis RJ]] |
| - | [[Category: Biofilm]] | + | |
| - | [[Category: Dna]] | + | |
| - | [[Category: Endonuclease]] | + | |
| - | [[Category: Hydrolase]]
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| Structural highlights
Function
F1BV52_BACLI
Publication Abstract from PubMed
Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a marine strain of Bacillus licheniformis, and present its crystal structure together with the biochemistry and a mutational analysis required to confirm its active site. Taken together, these data support the categorization of NucB into a unique subfamily of the betabetaalpha metal-dependent non-specific endonucleases. Understanding the structure and function of NucB will facilitate its future development into an anti-biofilm therapeutic agent.
Crystal structure of NucB, a biofilm-degrading endonuclease.,Basle A, Hewitt L, Koh A, Lamb HK, Thompson P, Burgess JG, Hall MJ, Hawkins AR, Murray H, Lewis RJ Nucleic Acids Res. 2017 Nov 20. pii: 4641913. doi: 10.1093/nar/gkx1170. PMID:29165717[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Basle A, Hewitt L, Koh A, Lamb HK, Thompson P, Burgess JG, Hall MJ, Hawkins AR, Murray H, Lewis RJ. Crystal structure of NucB, a biofilm-degrading endonuclease. Nucleic Acids Res. 2017 Nov 20. pii: 4641913. doi: 10.1093/nar/gkx1170. PMID:29165717 doi:http://dx.doi.org/10.1093/nar/gkx1170
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