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| | <StructureSection load='5ooo' size='340' side='right'caption='[[5ooo]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='5ooo' size='340' side='right'caption='[[5ooo]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ooo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OOO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OOO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ooo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rift_Valley_fever_virus Rift Valley fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OOO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ooo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ooo OCA], [http://pdbe.org/5ooo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ooo RCSB], [http://www.ebi.ac.uk/pdbsum/5ooo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ooo ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ooo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ooo OCA], [https://pdbe.org/5ooo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ooo RCSB], [https://www.ebi.ac.uk/pdbsum/5ooo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ooo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/NSS_RVFVZ NSS_RVFVZ] Inhibits host general transcription by inhibiting host basal transcription factor TFIIH.<ref>PMID:21543505</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Barski, M S]] | + | [[Category: Rift Valley fever virus]] |
| - | [[Category: Potter, J A]] | + | [[Category: Barski MS]] |
| - | [[Category: Schwarz-Linek, U]] | + | [[Category: Potter JA]] |
| - | [[Category: Interferon antagonist]] | + | [[Category: Schwarz-Linek U]] |
| - | [[Category: Non-structural protein]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| - | [[Category: Virulence factor]]
| + | |
| Structural highlights
Function
NSS_RVFVZ Inhibits host general transcription by inhibiting host basal transcription factor TFIIH.[1]
Publication Abstract from PubMed
Rift Valley fever phlebovirus (RVFV) is a clinically and economically important pathogen increasingly likely to cause widespread epidemics. RVFV virulence depends on the interferon antagonist non-structural protein (NSs), which remains poorly characterized. We identified a stable core domain of RVFV NSs (residues 83-248), and solved its crystal structure, a novel all-helical fold organized into highly ordered fibrils. A hallmark of RVFV pathology is NSs filament formation in infected cell nuclei. Recombinant virus encoding the NSs core domain induced intranuclear filaments, suggesting it contains all essential determinants for nuclear translocation and filament formation. Mutations of key crystal fibril interface residues in viruses encoding full-length NSs completely abrogated intranuclear filament formation in infected cells. We propose the fibrillar arrangement of the NSs core domain in crystals reveals the molecular basis of assembly of this key virulence factor in cell nuclei. Our findings have important implications for fundamental understanding of RVFV virulence.
Rift Valley fever phlebovirus NSs protein core domain structure suggests molecular basis for nuclear filaments.,Barski M, Brennan B, Miller OK, Potter JA, Vijayakrishnan S, Bhella D, Naismith JH, Elliott RM, Schwarz-Linek U Elife. 2017 Sep 15;6. pii: e29236. doi: 10.7554/eLife.29236. PMID:28915104[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kalveram B, Lihoradova O, Ikegami T. NSs protein of rift valley fever virus promotes posttranslational downregulation of the TFIIH subunit p62. J Virol. 2011 Jul;85(13):6234-43. doi: 10.1128/JVI.02255-10. Epub 2011 May 4. PMID:21543505 doi:http://dx.doi.org/10.1128/JVI.02255-10
- ↑ Barski M, Brennan B, Miller OK, Potter JA, Vijayakrishnan S, Bhella D, Naismith JH, Elliott RM, Schwarz-Linek U. Rift Valley fever phlebovirus NSs protein core domain structure suggests molecular basis for nuclear filaments. Elife. 2017 Sep 15;6. pii: e29236. doi: 10.7554/eLife.29236. PMID:28915104 doi:http://dx.doi.org/10.7554/eLife.29236
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