8sj5
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Walnut Tree Phytocystatin== | |
| + | <StructureSection load='8sj5' size='340' side='right'caption='[[8sj5]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8sj5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Juglans_regia Juglans regia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SJ5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8sj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8sj5 OCA], [https://pdbe.org/8sj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8sj5 RCSB], [https://www.ebi.ac.uk/pdbsum/8sj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8sj5 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A2I4FN75_JUGRE A0A2I4FN75_JUGRE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Biotechnological applications of phytocystatins have garnered significant interest due to their potential applications in crop protection and improve crop resistance to abiotic stress factors. Cof1 and Wal1 are phytocystatins derived from Coffea arabica and Juglans regia, respectively. These plants hold significant economic value due to coffee's global demand and the walnut tree's production of valuable timber and widely consumed walnuts with culinary and nutritional benefits. The study involved the heterologous expression in E. coli Lemo 21(DE3), purification by immobilized metal ion affinity and size exclusion chromatography, and biophysical characterization of both phytocystatins, focusing on isolating and interconverting their monomers and dimers. The crystal structure of the domain-swapped dimer of Wal1 was determined revealing two domain-swapped dimers in the asymmetric unit, an arrangement reminiscent of the human cystatin C structure. Alphafold models of monomers and Alphafold-Multimer models of domain-swapped dimers of Cof1 and Wal1 were analyzed in the context of the crystal structure. The methodology and data presented here contribute to a deeper understanding of the oligomerization mechanisms of phytocystatins and their potential biotechnological applications in agriculture. | ||
| - | + | Crystal structure and interconversion of monomers and domain-swapped dimers of the walnut tree phytocystatin.,Simpson GA, Rezende IF, da Silva Peixoto A, de Oliveira Soares IB, Barbosa JARG, de Freitas SM, Valadares NF Biochim Biophys Acta Proteins Proteom. 2023 Dec 12;1872(2):140975. doi: , 10.1016/j.bbapap.2023.140975. PMID:38056804<ref>PMID:38056804</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Valadares | + | <div class="pdbe-citations 8sj5" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Juglans regia]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Valadares NF]] | ||
Current revision
Walnut Tree Phytocystatin
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