1p2s

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[[Image:1p2s.jpg|left|200px]]
[[Image:1p2s.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1p2s |SIZE=350|CAPTION= <scene name='initialview01'>1p2s</scene>, resolution 2.45&Aring;
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The line below this paragraph, containing "STRUCTURE_1p2s", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ETF:TRIFLUOROETHANOL'>ETF</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= HRAS OR HRAS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1p2s| PDB=1p2s | SCENE= }}
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|RELATEDENTRY=[[1p2t|1P2T]], [[1p2u|1P2U]], [[1p2v|1P2V]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p2s OCA], [http://www.ebi.ac.uk/pdbsum/1p2s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p2s RCSB]</span>
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}}
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'''H-Ras 166 in 50% 2,2,2 triflouroethanol'''
'''H-Ras 166 in 50% 2,2,2 triflouroethanol'''
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[[Category: Mattos, C.]]
[[Category: Mattos, C.]]
[[Category: Serrano, V de.]]
[[Category: Serrano, V de.]]
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[[Category: molecular switch protein]]
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[[Category: Molecular switch protein]]
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[[Category: signaling protein]]
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[[Category: Signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:36:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:54:02 2008''
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Revision as of 01:36, 3 May 2008

Image:1p2s.jpg

Template:STRUCTURE 1p2s

H-Ras 166 in 50% 2,2,2 triflouroethanol


Overview

Room temperature crystal structures of crosslinked H-Ras bound to GMPPNP were solved in 50% 2,2,2-trifluoroethanol, 60% 1,6-hexanediol, and 50% isopropanol. The disordered switch II region of Ras is ordered in the presence of 2,2,2-trifluoroethanol or 1,6-hexanediol. The overall backbone conformation of switch II in these organic solvents is the same as in the Ras-GMPPNP complexes with RalGDS, PI(3) kinase, and RasGAP, indicating a biologically relevant form. Key polar interactions that stabilize the ordered switch are enhanced in the presence of hydrophobic cosolvents. These results suggest that hydrophobic solvents can be used in general to order short biologically relevant segments of disordered regions in protein crystals by favoring H-bonding interactions between atoms that are highly solvated and mobile in aqueous solution.

About this Structure

1P2S is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Organic solvents order the dynamic switch II in Ras crystals., Buhrman G, de Serrano V, Mattos C, Structure. 2003 Jul;11(7):747-51. PMID:12842038 Page seeded by OCA on Sat May 3 04:36:39 2008

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