2jqt

From Proteopedia

(Difference between revisions)

Current revision

Structure of the bacterial replication origin-associated protein Cnu

Structural highlights

2jqt is a 1 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNU_ECOLI The complex formed with H-NS binds to the specific 26-bp cnb site in the origin of replication oriC. Can complement, at least partially, the absence of the Hha protein in hha mutants.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cnu is a nucleoid protein that has a high degree of sequence homology with Hha/YmoA family proteins, which bind to chromatin and regulate the expression of Escherichia coli virulence genes in response to changes in temperature or ionic strength. Here, we determined its solution structure and dynamic properties and mapped H-NS binding sites. Cnu consists of three alpha helices that are comparable with those of Hha, but it has significant flexibility in the C-terminal region and lacks a short alpha helix present in Hha. Upon increasing ionic strength, the helical structure of Cnu is destabilized, especially at the ends of the helices. The dominant H-NS binding sites, located at helix 3 as in Hha, reveal a common structural platform for H-NS binding. Our results may provide structural and dynamic bases for the similarity and dissimilarity between Cnu and Hha functions.

Structure of the nucleoid-associated protein Cnu reveals common binding sites for H-NS in Cnu and Hha.,Bae SH, Liu D, Lim HM, Lee Y, Choi BS Biochemistry. 2008 Feb 19;47(7):1993-2001. Epub 2008 Jan 12. PMID:18189420^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kim MS, Bae SH, Yun SH, Lee HJ, Ji SC, Lee JH, Srivastava P, Lee SH, Chae H, Lee Y, Choi BS, Chattoraj DK, Lim HM. Cnu, a novel oriC-binding protein of Escherichia coli. J Bacteriol. 2005 Oct;187(20):6998-7008. PMID:16199570 doi:http://dx.doi.org/10.1128/JB.187.20.6998-7008.2005
↑ Bae SH, Liu D, Lim HM, Lee Y, Choi BS. Structure of the nucleoid-associated protein Cnu reveals common binding sites for H-NS in Cnu and Hha. Biochemistry. 2008 Feb 19;47(7):1993-2001. Epub 2008 Jan 12. PMID:18189420 doi:10.1021/bi701914t

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jqt"

@@ Line 1: / Line 1: @@
 ==Structure of the bacterial replication origin-associated protein Cnu==
-<StructureSection load='2jqt' size='340' side='right'caption='[[2jqt]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2jqt' size='340' side='right'caption='[[2jqt]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jqt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JQT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jqt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JQT FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jw2|1jw2]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ydgT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jqt OCA], [https://pdbe.org/2jqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jqt RCSB], [https://www.ebi.ac.uk/pdbsum/2jqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jqt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CNU_ECOLI CNU_ECOLI]] The complex formed with H-NS binds to the specific 26-bp cnb site in the origin of replication oriC. Can complement, at least partially, the absence of the Hha protein in hha mutants.<ref>PMID:16199570</ref>
+[https://www.uniprot.org/uniprot/CNU_ECOLI CNU_ECOLI] The complex formed with H-NS binds to the specific 26-bp cnb site in the origin of replication oriC. Can complement, at least partially, the absence of the Hha protein in hha mutants.<ref>PMID:16199570</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Bae, S H]]
+[[Category: Bae SH]]
-[[Category: Choi, B S]]
+[[Category: Choi BS]]
-[[Category: Lee, Y]]
+[[Category: Lee Y]]
-[[Category: Lim, H M]]
+[[Category: Lim HM]]
-[[Category: Liu, D]]
+[[Category: Liu D]]
-[[Category: Cnu]]
-[[Category: H-n]]
-[[Category: Oric]]
-[[Category: Protein binding]]
-[[Category: Replication origin associated]]
-[[Category: Ydgt]]

2jqt

From Proteopedia

Current revision

Structure of the bacterial replication origin-associated protein Cnu

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox