1p42
From Proteopedia
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'''Crystal structure of Aquifex aeolicus LpxC Deacetylase (Zinc-Inhibited Form)''' | '''Crystal structure of Aquifex aeolicus LpxC Deacetylase (Zinc-Inhibited Form)''' | ||
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[[Category: Shin, H.]] | [[Category: Shin, H.]] | ||
[[Category: Whittington, D A.]] | [[Category: Whittington, D A.]] | ||
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| - | [[Category: | + | [[Category: Hydrophobic tunnel]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:39:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:39, 3 May 2008
Crystal structure of Aquifex aeolicus LpxC Deacetylase (Zinc-Inhibited Form)
Overview
The outer leaflet of the outer membrane of the Gram-negative bacterium serves as a permeability barrier and is composed of lipopolysaccharide, also known as endotoxin. The membrane anchor of lipopolysaccharide is lipid A, the biosynthesis of which is essential for cell viability. The first committed step in lipid A biosynthesis is catalyzed by UDP-(3-O-(R-3-hydroxymyristoyl))-N-acetylglucosamine deacetylase (LpxC), a zinc-dependent deacetylase. Here we report the crystal structure of LpxC from Aquifex aeolicus, which reveals a new alpha+beta fold reflecting primordial gene duplication and fusion, as well as a new zinc-binding motif. The catalytic zinc ion resides at the base of an active-site cleft and adjacent to a hydrophobic tunnel occupied by a fatty acid. This tunnel accounts for the specificity of LpxC toward substrates and inhibitors bearing appropriately positioned 3-O-fatty acid substituents. Notably, simple inhibitors designed to target interactions in the hydrophobic tunnel bind with micromolar affinity, thereby representing a step toward the structure-based design of a potent, broad-spectrum antibacterial drug.
About this Structure
1P42 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of LpxC, a zinc-dependent deacetylase essential for endotoxin biosynthesis., Whittington DA, Rusche KM, Shin H, Fierke CA, Christianson DW, Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8146-50. Epub 2003 Jun 20. PMID:12819349 Page seeded by OCA on Sat May 3 04:39:20 2008
